Universität zu Köln

Nussey, Daniel H., Baird, Duncan, Barrett, Emma, Boner, Winnie, Fairlie, Jennifer, Gemmell, Neil, Hartmann, Nils, Horn, Thorsten, Haussmann, Mark, Olsson, Mats ORCID: 0000-0002-4130-1323, Turbill, Chris, Verhulst, Simon ORCID: 0000-0002-1143-6868, Zahn, Sandrine and Monaghan, Pat (2014). Measuring telomere length and telomere dynamics in evolutionary biology and ecology. Methods Ecol. Evol., 5 (4). S. 299 - 311. HOBOKEN: WILEY. ISSN 2041-2096

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Abstract

Telomeres play a fundamental role in the protection of chromosomal DNA and in the regulation of cellular senescence. Recent work in human epidemiology and evolutionary ecology suggests adult telomere length (TL) may reflect past physiological stress and predict subsequent morbidity and mortality, independent of chronological age. Several different methods have been developed to measure TL, each offering its own technical challenges. The aim of this review is to provide an overview of the advantages and drawbacks of each method for researchers, with a particular focus on issues that are likely to face ecologists and evolutionary biologists collecting samples in the field or in organisms that may never have been studied in this context before. We discuss the key issues to consider and wherever possible try to provide current consensus view regarding best practice with regard to sample collection and storage, DNA extraction and storage, and the five main methods currently available to measure TL. Decisions regarding which tissues to sample, how to store them, how to extract DNA, and which TL measurement method to use cannot be prescribed, and are dependent on the biological question addressed and the constraints imposed by the study system. What is essential for future studies of telomere dynamics in evolution and ecology is that researchers publish full details of their methods and the quality control thresholds they employ.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nussey, Daniel H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baird, Duncan UNSPECIFIED UNSPECIFIED UNSPECIFIED Barrett, Emma UNSPECIFIED UNSPECIFIED UNSPECIFIED Boner, Winnie UNSPECIFIED UNSPECIFIED UNSPECIFIED Fairlie, Jennifer UNSPECIFIED UNSPECIFIED UNSPECIFIED Gemmell, Neil UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Nils UNSPECIFIED UNSPECIFIED UNSPECIFIED Horn, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Haussmann, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Olsson, Mats UNSPECIFIED orcid.org/0000-0002-4130-1323 UNSPECIFIED Turbill, Chris UNSPECIFIED UNSPECIFIED UNSPECIFIED Verhulst, Simon UNSPECIFIED orcid.org/0000-0002-1143-6868 UNSPECIFIED Zahn, Sandrine UNSPECIFIED UNSPECIFIED UNSPECIFIED Monaghan, Pat UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-442763
DOI:	10.1111/2041-210X.12161
Journal or Publication Title:	Methods Ecol. Evol.
Volume:	5
Number:	4
Page Range:	S. 299 - 311
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	2041-2096
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language QUANTITATIVE PCR; LONGITUDINAL FINDINGS; OXIDATIVE STRESS; BASE-LINE; AGE; SEQUENCES; HETEROGENEITY; DETERMINANTS; VARIABILITY; REGRESSION Multiple languages Ecology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44276