Universität zu Köln

Zhang, Lijun, Thevis, Mario, Piper, Thomas ORCID: 0000-0002-7462-6693, Jochmann, Maik A., Wolbert, J. Benjamin, Kujawinski, Dorothea M., Wiese, Steffen, Teutenberg, Thorsten and Schmidt, Torsten C. (2014). Carbon Isotope Ratio Analysis of Steroids by High-Temperature Liquid Chromatography-Isotope Ratio Mass Spectrometry. Anal. Chem., 86 (5). S. 2297 - 2303. WASHINGTON: AMER CHEMICAL SOC. ISSN 1520-6882

Full text not available from this repository.

Abstract

Generally, compound-specific isotope analysis of steroids is carried out by gas chromatography combined with isotope ratio mass spectrometry. Thus, a derivatization of the steroids prior to the measurement is compulsory, and a correction of the isotopic data is often necessary. To overcome this limitation, we present a new approach of high-temperature liquid chromatography coupled with photodiode array detection and isotope ratio mass spectrometry (HT-LC/PDA/IRMS) for the carbon isotope ratio analysis of unconjugated steroids. A steroid mixture containing 19-norandrosterone, testosterone, epitestosterone, androsterone, and 5 beta-pregnane-3 alpha,17 alpha,20 alpha-triol was fully separated on a C-4 column under high-temperature elution with water as the sole eluent. The accuracy for isotope analysis (+/- 0.5 parts per thousand) was around 20 mu g g(-1) for testosterone, epitestosterone (79 ng steroid absolute on column), and 30 mu g g(-1) for 19-norandrosterone, androsterone, and 5 beta-pregnane-3 alpha,17 alpha,20 alpha-triol (119 ng steroid absolute on column). The applicability of the method was tested by measuring a pharmaceutical gel containing testosterone. With this work, the scope of LC/IRMS applications has been extended to nonpolar compounds.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhang, Lijun UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Piper, Thomas UNSPECIFIED orcid.org/0000-0002-7462-6693 UNSPECIFIED Jochmann, Maik A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolbert, J. Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Kujawinski, Dorothea M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiese, Steffen UNSPECIFIED UNSPECIFIED UNSPECIFIED Teutenberg, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Torsten C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-443552
DOI:	10.1021/ac403353x
Journal or Publication Title:	Anal. Chem.
Volume:	86
Number:	5
Page Range:	S. 2297 - 2303
Date:	2014
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1520-6882
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DOPING CONTROL; WATER CHROMATOGRAPHY; STATIONARY PHASES; ORGANIC-COMPOUNDS; C-13/C-12 RATIOS; REVERSED-PHASE; TESTOSTERONE; CATTLE; DERIVATIZATION; INTERFACE Multiple languages Chemistry, Analytical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44355