Universität zu Köln

Foesel, Baerbel U., Naegele, Verena, Naether, Astrid, Wuest, Pia K., Weinert, Jan, Bonkowski, Michael ORCID: 0000-0003-2656-1183, Lohaus, Gertrud, Polle, Andrea ORCID: 0000-0001-8697-6394, Alt, Fabian, Oelmann, Yvonne ORCID: 0000-0003-3513-6568, Fischer, Markus ORCID: 0000-0002-5589-5900, Friedrich, Michael W. ORCID: 0000-0002-8055-3232 and Overmann, Joerg (2014). Determinants of Acidobacteria activity inferred from the relative abundances of 16S rRNA transcripts in German grassland and forest soils. Environ. Microbiol., 16 (3). S. 658 - 676. HOBOKEN: WILEY. ISSN 1462-2920

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Abstract

16S rRNA genes and transcripts of Acidobacteria were investigated in 57 grassland and forest soils of three different geographic regions. Acidobacteria contributed 9-31% of bacterial 16S rRNA genes whereas the relative abundances of the respective transcripts were 4-16%. The specific cellular 16S rRNA content (determined as molar ratio of rRNA:rRNA genes) ranged between 3 and 80, indicating a low in situ growth rate. Correlations with flagellate numbers, vascular plant diversity and soil respiration suggest that biotic interactions are important determinants of Acidobacteria 16S rRNA transcript abundances in soils. While the phylogenetic composition of Acidobacteria differed significantly between grassland and forest soils, high throughput denaturing gradient gel electrophoresis and terminal restriction fragment length polymorphism fingerprinting detected 16S rRNA transcripts of most phylotypes in situ. Partial least squares regression suggested that chemical soil conditions such as pH, total nitrogen, C:N ratio, ammonia concentrations and total phosphorus affect the composition of this active fraction of Acidobacteria. Transcript abundance for individual Acidobacteria phylotypes was found to correlate with particular physicochemical (pH, temperature, nitrogen or phosphorus) and, most notably, biological parameters (respiration rates, abundances of ciliates or amoebae, vascular plant diversity), providing culture-independent evidence for a distinct niche specialization of different Acidobacteria even from the same subdivision.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Foesel, Baerbel U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Naegele, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Naether, Astrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Wuest, Pia K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Weinert, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonkowski, Michael UNSPECIFIED orcid.org/0000-0003-2656-1183 UNSPECIFIED Lohaus, Gertrud UNSPECIFIED UNSPECIFIED UNSPECIFIED Polle, Andrea UNSPECIFIED orcid.org/0000-0001-8697-6394 UNSPECIFIED Alt, Fabian UNSPECIFIED UNSPECIFIED UNSPECIFIED Oelmann, Yvonne UNSPECIFIED orcid.org/0000-0003-3513-6568 UNSPECIFIED Fischer, Markus UNSPECIFIED orcid.org/0000-0002-5589-5900 UNSPECIFIED Friedrich, Michael W. UNSPECIFIED orcid.org/0000-0002-8055-3232 UNSPECIFIED Overmann, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-445249
DOI:	10.1111/1462-2920.12162
Journal or Publication Title:	Environ. Microbiol.
Volume:	16
Number:	3
Page Range:	S. 658 - 676
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1462-2920
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OPERON COPY NUMBER; BACTERIAL COMMUNITY; PHYLUM ACIDOBACTERIA; GEN. NOV.; MICROBIAL COMMUNITY; DIVERSITY; MEMBERS; GROWTH; CULTIVATION; SOFTWARE Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44524