Universität zu Köln

Salscheider, Silja Lucia, Jahn, Andreas and Schnetz, Karin ORCID: 0000-0003-4693-6202 (2014). Transcriptional regulation by BglJ-RcsB, a pleiotropic heteromeric activator in Escherichia coli. Nucleic Acids Res., 42 (5). S. 2999 - 3009. OXFORD: OXFORD UNIV PRESS. ISSN 1362-4962

Full text not available from this repository.

Abstract

The bacterial Rcs phosphorelay signals perturbations of the bacterial cell envelope to its response regulator RcsB, which regulates transcription of multiple loci related to motility, biofilm formation and various stress responses. RcsB is unique, as its set of target loci is modulated by interaction with auxiliary regulators including BglJ. The BglJRcsB heteromer is known to activate the HNS repressed leuO and bgl loci independent of RcsB phosphorylation. Here, we show that BglJ-RcsB activates the promoters of 10 additional loci (chiA, molR, sfsB, yecT, yqhG, ygiZ, yidL, ykiA, ynbA and ynjI). Furthermore, we mapped the BglJ-RcsB binding site at seven loci and propose a consensus sequence motif. The data suggest that activation by BglJ-RcsB is DNA phasing dependent at some loci, a feature reminiscent of canonical transcriptional activators, while at other loci BglJ-RcsB activation may be indirect by inhibition of HNS-mediated repression. In addition, we show that BglJ-RcsB activates transcription of bgl synergistically with CRP where it shifts the transcription start by 20 bp from a position typical for class I CRP-dependent promoters to a position typical for class II CRP-dependent promoters. Thus, BglJ-RcsB is a pleiotropic transcriptional activator that coordinates regulation with global regulators including CRP, LeuO and HNS.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Salscheider, Silja Lucia UNSPECIFIED UNSPECIFIED UNSPECIFIED Jahn, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnetz, Karin UNSPECIFIED orcid.org/0000-0003-4693-6202 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-445554
DOI:	10.1093/nar/gkt1298
Journal or Publication Title:	Nucleic Acids Res.
Volume:	42
Number:	5
Page Range:	S. 2999 - 3009
Date:	2014
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1362-4962
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AMP RECEPTOR PROTEIN; H-NS; ENTERIC BACTERIA; STRESS-RESPONSE; RNA-POLYMERASE; OPERON; GENE; PROMOTER; PHOSPHORELAY; K-12 Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/44555