Universität zu Köln

Schlereth, Simona L., Neuser, Barbara, Caramoy, Albert, Grajewski, Rafael S., Koch, Konrad R., Schroedl, Falk, Cursiefen, Claus and Heindl, Ludwig M. (2014). Enrichment of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1)-Positive Macrophages Around Blood Vessels in the Normal Human Sclera. Invest. Ophthalmol. Vis. Sci., 55 (2). S. 865 - 873. ROCKVILLE: ASSOC RESEARCH VISION OPHTHALMOLOGY INC. ISSN 1552-5783

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Abstract

PURPOSE. To investigate whether the normal adult human sclera contains lymphatic vessels and to study their relation to immune cells and blood vessel anatomy. METHODS. Scleral tissue probes from 35 adult human donor bulbi were analyzed by immunohistochemistry and confocal microscopy for blood vessels (CD31+), lymphatic vessels (lymphatic vessel endothelial hyaluronan receptor 1 [LYVE1] +, podoplanin+), and macrophages (CD68+) at 12 locations (anterior, equatorial, and posterior at 3, 6, 9, and 12 o'clock positions of the eye) in all three scleral layers (episclera, stroma, and lamina fusca). Approval for scientific examination was obtained. RESULTS. CD31+ blood vessels were detectable in the human sclera, where the percentage area covered by CD31+ blood vessels was highest in the anterior episclera, followed by equatorial and posterior episclera, and was lowest in the scleral stroma (regardless of location). LYVE1+ podoplanin+ lymphatic vessels were not detectable in any location investigated, although there was a high number of LYVE1+ CD68+ macrophages. These macrophages were concentrated around blood vessels. In contrast, in the episclera, the number of detected LYVE1+ CD68+ macrophages was comparable in all locations; within the stroma, their number increased toward the posterior part of the eye. CONCLUSIONS. The adult sclera contains blood vessels but lacks, as revealed by immunohistochemistry and confocal microscopy, true lymphatic vessels. LYVE1+ CD68+ macrophages are located adjacent to the longitudinal axis of blood vessels. The function of these cells needs further investigation, but could be a next step toward a better understanding of pathological disorders such as inflammation, tumor, trauma, or glaucoma.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schlereth, Simona L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Neuser, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Caramoy, Albert UNSPECIFIED UNSPECIFIED UNSPECIFIED Grajewski, Rafael S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Konrad R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroedl, Falk UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-446842
DOI:	10.1167/iovs.13-13453
Journal or Publication Title:	Invest. Ophthalmol. Vis. Sci.
Volume:	55
Number:	2
Page Range:	S. 865 - 873
Date:	2014
Publisher:	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Place of Publication:	ROCKVILLE
ISSN:	1552-5783
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TUMOR-ASSOCIATED LYMPHANGIOGENESIS; CILIARY-BODY-MELANOMAS; EXTRAOCULAR-EXTENSION; MALIGNANT MELANOMAS; BREAST-CANCER; CORNEAL; ANGIOGENESIS; PODOPLANIN; TRANSPLANTATION; HEMANGIOGENESIS Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44684