Universität zu Köln

Koenig, Sandy, Luheshi, Giamal N., Wenz, Tina, Gerstberger, Ruediger, Roth, Joachim and Rummel, Christoph (2014). Leptin is involved in age-dependent changes in response to systemic inflammation in the rat. Brain Behav. Immun., 36. S. 128 - 139. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2139

Full text not available from this repository.

Abstract

Obesity contributes to a state of subclinical peripheral and central inflammation and is often associated with aging. Here we investigated the source and contribution of adipose tissue derived cytokines and the cytokine-like hormone leptin to age-related changes in lipopolysaccharide (LPS)-induced brain-controlled sickness-responses. Old (24 months) and young (2 months) rats were challenged with LPS or saline alone or in combination with a neutralizing leptin antiserum (LAS) or control serum. Changes in the sickness-response were monitored by biotelemetry. Additionally, ex vivo fat-explants from young and old rats were stimulated with LPS or saline and culture medium collected and analyzed by cytokine-specific bioassays/ELISAs. We found enhanced duration/degree of the sickness-symptoms, including delayed but prolonged fever in old rats. This response was accompanied by increased plasma-levels of interleukin (IL)-6 and IL-1ra and exaggerated expression of inflammatory markers in brain and liver analyzed by RTPCR including inhibitor kappa B alpha, microsomal prostaglandin synthase and cyclooxygenase 2 (brain). Moreover, for the first time, we were able to show prolonged elevated plasma leptin-levels in LPS-treated old animals. Treatment with LAS in young rats tended to attenuate the early- and in old rats the prolonged febrile response. Fat-explants exhibited unchanged IL-6 but reduced IL-1ra and tumor necrosis factor (TNE)-alpha release from adipose tissue of aged compared to young animals. In addition, we found increased expression of the endogenous immune regulator microRNA146a in aged animals suggesting a role for these mediators in counteracting brain inflammation. Overall, our results indicate a role of adipose tissue and leptin in aging-related-inflammation and age-dependent modifications of febrile-responses. (C) 2013 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koenig, Sandy UNSPECIFIED UNSPECIFIED UNSPECIFIED Luheshi, Giamal N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wenz, Tina UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerstberger, Ruediger UNSPECIFIED UNSPECIFIED UNSPECIFIED Roth, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED Rummel, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-447341
DOI:	10.1016/j.bbi.2013.10.019
Journal or Publication Title:	Brain Behav. Immun.
Volume:	36
Page Range:	S. 128 - 139
Date:	2014
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2139
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LIPOPOLYSACCHARIDE-INDUCED FEVER; RESPIRATORY FACTOR-I; SICKNESS BEHAVIOR; PROINFLAMMATORY CYTOKINES; ADIPOSE-TISSUE; IMMUNE-SYSTEM; BRAIN; EXPRESSION; INTERLEUKIN-6; ACTIVATION Multiple languages Immunology; Neurosciences; Psychiatry Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44734