Universität zu Köln

Kaur, Jaspreet, Schildgen, Verena, Tillmann, Ramona-Liza, Hardt, Anna-Lena, Luesebrink, Jessica, Windisch, Wolfram and Schildgen, Oliver ORCID: 0000-0003-4297-9627 (2014). Low copy number detection of human bocavirus DNA in bronchoalveolar lavage of asymptomatic adult patients. Future Virol., 9 (8). S. 715 - 721. LONDON: FUTURE MEDICINE LTD. ISSN 1746-0808

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Abstract

Aim: The aim of this study was to investigate the percentage of such asymptomatic human bocavirus (HBoV) DNA-shedding patients with a sensitive commercial method. Materials & methods: Bronchoalveolar lavage fluids were analyzed with the xTag (R) respiratory viral panel (RVP) assay (Luminex, The Netherlands), the RespiFinder (R) SMART assay (PathoFinder, Maastricht, The Netherlands) and an in-house real-time PCR protocol. Results: While replacing the Luminex xTag RVP assay with the RespiFinder SMART assay for routine diagnostics, surprisingly, more than 50% of bronchoalveolar lavage fluid samples from asymptomatic patients previously tested with negative Luminex xTag RVP tested positive for HBoV. Conclusion: As the RespiFinder SMART assay has a higher sensitivity in the present study, this supports the theory that HBoV persists and is capable of shedding low copy numbers of viral genomes at very low numbers, even in asymptomatic people.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kaur, Jaspreet UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Tillmann, Ramona-Liza UNSPECIFIED UNSPECIFIED UNSPECIFIED Hardt, Anna-Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Luesebrink, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Windisch, Wolfram UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-449449
DOI:	10.2217/FVL.14.59
Journal or Publication Title:	Future Virol.
Volume:	9
Number:	8
Page Range:	S. 715 - 721
Date:	2014
Publisher:	FUTURE MEDICINE LTD
Place of Publication:	LONDON
ISSN:	1746-0808
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REAL-TIME PCR; CHILDREN; ASSAY Multiple languages Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44944