Universität zu Köln

Andereggen, Lukas ORCID: 0000-0003-1764-688X, Neuschmelting, Volker ORCID: 0000-0001-7527-6990, von Gunten, Michael, Widmer, Hans Rudolf ORCID: 0000-0003-3378-8765, Fandino, Javier and Marbacher, Serge ORCID: 0000-0001-6305-7571 (2014). The Role of Microclot Formation in an Acute Subarachnoid Hemorrhage Model in the Rabbit. Biomed Res. Int., 2014. LONDON: HINDAWI LTD. ISSN 2314-6141

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Abstract

Background. Microvascular dysfunction and microthrombi formation are believed to contribute to development of early brain injury (EBI) after aneurysmal subarachnoid hemorrhage (SAH). Objective. This study aimed to determine (i) extent of microthrombus formation and neuronal apoptosis in the brain parenchyma using a blood shunt SAH model in rabbits; (ii) correlation of structural changes in microvessels with EBI characteristics. Methods. Acute SAH was induced using a rabbit shunt cisterna magna model. Extent of microthrombosis was detected 24 h post-SAH (n = 8) by fibrinogen immunostaining, compared to controls (n = 4). We assessed apoptosis by terminal deoxynucleotidyl transferase nick end labeling (TUNEL) in cortex and hippocampus. Results. Our results showed significantly more TUNEL-positive cells (SAH: 115 +/- 13; controls: 58 +/- 10; P = 0.016) and fibrinogen-positive microthromboemboli (SAH: 9 +/- 2; controls: 2 +/- 1;P = 0.03) in the hippocampus after aneurysmal SAH. Conclusions. We found clear evidence of early microclot formation in a rabbit model of acute SAH. The extent of microthrombosis did not correlate with early apoptosis or CPP depletion after SAH; however, the total number of TUNEL positive cells in the cortex and the hippocampus significantly correlated with mean CPP reduction during the phase of maximum depletion after SAH induction. Both microthrombosis and neuronal apoptosis may contribute to EBI and subsequent DCI.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Andereggen, Lukas UNSPECIFIED orcid.org/0000-0003-1764-688X UNSPECIFIED Neuschmelting, Volker UNSPECIFIED orcid.org/0000-0001-7527-6990 UNSPECIFIED von Gunten, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Widmer, Hans Rudolf UNSPECIFIED orcid.org/0000-0003-3378-8765 UNSPECIFIED Fandino, Javier UNSPECIFIED UNSPECIFIED UNSPECIFIED Marbacher, Serge UNSPECIFIED orcid.org/0000-0001-6305-7571 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-449940
DOI:	10.1155/2014/161702
Journal or Publication Title:	Biomed Res. Int.
Volume:	2014
Date:	2014
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	2314-6141
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EARLY BRAIN-INJURY; GLOBAL CEREBRAL ATROPHY; INTRACRANIAL-PRESSURE; ANIMAL-MODELS; BLOOD-FLOW; MICROTHROMBOSIS; MECHANISMS; CONSTRICTION; INFLAMMATION; COAGULATION Multiple languages Biotechnology & Applied Microbiology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44994