Universität zu Köln

Kapisch, Iwona, Silling, Steffi, Brockmann, Michael, Schildgen, Oliver ORCID: 0000-0003-4297-9627, Tillmann, Ramona-Liza and Schildgen, Verena (2014). Comparison of molecular multiplex assays for the detection of human papillomaviruses from clinical gynecological tissue samples. Future Virol., 9 (1). S. 21 - 27. LONDON: FUTURE MEDICINE LTD. ISSN 1746-0808

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Abstract

Papillomavirus-associated cervical cancer remains a major disease burden for young women. Therefore, it plays an important role in the pathological diagnosis. Besides cytological and classical pathological methods, molecular biology tests are used for the analysis of HPV status. Here we compare three PCR assays for the detection of human papillomavirus DNA from 50 gynecological samples, namely the HPV Genotyping Kit HPVsign(R) (Qiagen, Germany), a novel HPV-genotyping kit based on pyrosequencing, the LCD array HPV Type 3.5 (Chipron, Germany) and an in-house PCR of the National Reference Center for Papilloma-and Polyomaviruses at the Institute for Virology of the University Cologne (Germany). The HPVsign assay mainly detected the assumed leading HPV type, while both the in-house PCR and the Chipron HPV Assay 3.5 reproducibly detected single and multiple infections.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kapisch, Iwona UNSPECIFIED UNSPECIFIED UNSPECIFIED Silling, Steffi UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmann, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED Tillmann, Ramona-Liza UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-450372
DOI:	10.2217/FVL.13.119
Journal or Publication Title:	Future Virol.
Volume:	9
Number:	1
Page Range:	S. 21 - 27
Date:	2014
Publisher:	FUTURE MEDICINE LTD
Place of Publication:	LONDON
ISSN:	1746-0808
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HPV TYPES; DNA; INFECTIONS; PRIMERS Multiple languages Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/45037