Universität zu Köln

Isachenko, Vladimir, Rahimi, Gohar, Dattena, Maria, Mallmann, Peter, Baikoshkarova, Saltanat ORCID: 0000-0001-9428-489X, Kellerwessel, Elisabeth, Otarbaev, Marat ORCID: 0000-0002-4323-9252, Shalakhmetova, Tamara and Isachenko, Evgenia (2014). Whole Ovine Ovaries as a Model for Human: Perfusion with Cryoprotectants In Vivo and In Vitro. Biomed Res. Int., 2014. LONDON: HINDAWI LTD. ISSN 2314-6141

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Abstract

These experiments were performed to test the perfusion of ovine as a model for human ovaries by cryoprotectants in vivo at high temperature when the permeability of capillaries is high and when blood is insensibly replaced by the solution of cryoprotectants. By our hypothetical supposition, ovaries could be saturated by cryoprotectants before their surgical removal. The objective was to examine the effectiveness of perfusion of ovine ovaries with vascular pedicle in vivo and in vitro. Arteria ovarica was cannuled and ovaries were perfused by Leibovitz L-15 medium + 100 IU/mL heparin + 5% bovine calf serum +6% dimethyl sulfoxide +6% ethylene glycol + 0.15M sucrose + Indian ink in vivo and in vitro. In the first and second cycle of experiments, ovaries (n = 13 and n = 23) were perfused in vivo and in vitro, respectively, during 60 min with the rate of perfusion 50 mL/h (0.8 mL/min). It was established with in vivo perfusion that only about 10% of ovarian tissues were perfused due to an appearance of multiple anastomoses when the perfusion medium goes from arteria ovarica to arteria uterina without inflow into the ovaries. It was concluded that in vitro perfusion of ovine intact ovaries with vascular pedicle by freezing medium is more effective than this manipulation performed in vivo.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Isachenko, Vladimir UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Dattena, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Baikoshkarova, Saltanat UNSPECIFIED orcid.org/0000-0001-9428-489X UNSPECIFIED Kellerwessel, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Otarbaev, Marat UNSPECIFIED orcid.org/0000-0002-4323-9252 UNSPECIFIED Shalakhmetova, Tamara UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-450726
DOI:	10.1155/2014/409019
Journal or Publication Title:	Biomed Res. Int.
Volume:	2014
Date:	2014
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	2314-6141
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SHEEP OVARIES; TISSUE; CRYOPRESERVATION; TRANSPLANTATION; AUTOTRANSPLANTATION; VIABILITY; VITRIFICATION; AUTOGRAFTS; PREGNANCY; OOCYTES Multiple languages Biotechnology & Applied Microbiology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/45072