Universität zu Köln

Vent, J., Zimmermann, Ch., Drebber, U., Wedemeyer, I., Eckel, H. E., Huettenbrink, K. B. and Preuss, S. F. (2014). Influence of formalin fixation on tissue dimensions in palatal tonsils. Pathol. Res. Pract., 210 (1). S. 59 - 62. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG. ISSN 0344-0338

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Abstract

Aim: To investigate the change of tissue dimensions after formalin fixation, and to determine the optimal time of fixation. Hypothesis: Formalin fixation may lead to shrinkage in tissue dimensions and may thus alter tumor stages. Background: It is often observed in tumor surgery that the dimensions in vivo seem larger than after resection, and tissue appears to shrink further after formalin fixation. This might alter dimensions and assessment of spread of the tumor and thus lead to a lesser tumor classification and stage. In cases where the decision for adjuvant chemoradiation is based upon the stage, it may thus be of relevance for the patient to evaluate the pathologic and not the in vivo dimensions of the tumor. Material and methods: In order to obtain comparable tissues, we investigated 100 palatal tonsils after cold steel dissection tonsillectomy for chronic tonsillitis. There were four time points investigated: directly after excision in the operating room and after four, 24 and 72 h of fixation in formaldehyde (4% Formaldehyde in phosphate buffer pH 7.4). The tissue was measured in the following dimensions: volume (ml), weight (g) and length, broadness and width (mm). Results: The tissue size did not change significantly in dimensions except for an increase in length. The time of fixation did not influence the size. Discussion: Formalin fixation does not significantly influence the tissue dimensions of palatal tonsils in comparison to direct ex vivo measurements. A minimal time of fixation of 20 h is required in order to stop all degenerative processes; however, longer fixation does not change the dimensions of the specimen. Conclusion: The null hypothesis has to be withdrawn that tissue dimensions are altered by formalin fixation. Thus, the histopathological measurements do not influence TNM staging. 2013 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Vent, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zimmermann, Ch. UNSPECIFIED UNSPECIFIED UNSPECIFIED Drebber, U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wedemeyer, I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eckel, H. E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huettenbrink, K. B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Preuss, S. F. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-450962
DOI:	10.1016/j.prp.2013.10.002
Journal or Publication Title:	Pathol. Res. Pract.
Volume:	210
Number:	1
Page Range:	S. 59 - 62
Date:	2014
Publisher:	ELSEVIER GMBH, URBAN & FISCHER VERLAG
Place of Publication:	JENA
ISSN:	0344-0338
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NECK; HEAD Multiple languages Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/45096