Haas, C., Hanson, E., Anjos, M. J., Ballantyne, K. N., Banemann, R., Bhoelai, B., Borges, E., Carvalho, M., Courts, C., De Cock, G., Drobnic, K., Doetsch, M., Fleming, R., Franchi, C., Gomes, I., Hadzic, G., Harbison, S. A., Harteveld, J., Hjort, B., Hollard, C., Hoff-Olsen, P., Huels, C., Keyser, C., Maronas, O., McCallum, N., Moore, D., Morling, N., Niederstaetter, H., Noel, F., Parson, W., Phillips, C., Popielarz, C., Roeder, A. D., Salvaderi, L., Sauer, E., Schneider, P. M., Shanthan, G., Court, D. Syndercombe, Turanska, M., van Oorschot, R. A. H., Vennemann, M., Vidaki, A., Zatkalikova, L. and Ballantyne, J. (2014). RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise. Forensic Sci. Int.-Genet., 8 (1). S. 203 - 213. CLARE: ELSEVIER IRELAND LTD. ISSN 1878-0326

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Abstract

The European DNA Profiling Group (EDNAP) organized a fourth and fifth collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling. The task was to identify dried menstrual blood and vaginal secretion stains using specific RNA biomarkers, and additionally test 3 housekeeping genes for their suitability as reference genes. Six menstrual blood and six vaginal secretion stains, two dilution series (1/4-1/64 pieces of a menstrual blood/vaginal swab) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 24 participating laboratories, using RNA extraction or RNA/DNA co-extraction methods. Two novel menstrual blood mRNA multiplexes were used: MMP triplex (MMP7, MMP10, MMP11) and MB triplex (MSX1, LEFTY2, SFRP4) in conjunction with a housekeeping gene triplex (B2M, UBC, UCE). Two novel mRNA multiplexes and a HBD1 singleplex were used for the identification of vaginal secretion: Vag triplex (MYOZ1, CYP2B7P1 and MUC4) and a Lactobacillus-specific Lacto triplex (Ljen, Lcris, Lgas). The laboratories used different chemistries and instrumentation and all were able to successfully isolate and detect mRNA in dried stains. The simultaneous extraction of RNA and DNA allowed for positive identification of the tissue/fluid source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling, also from old and compromised casework samples. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid identification method that can easily be combined with current STR typing technology. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Haas, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hanson, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Anjos, M. J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ballantyne, K. N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Banemann, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bhoelai, B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Borges, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Carvalho, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Courts, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
De Cock, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Drobnic, K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doetsch, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fleming, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Franchi, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gomes, I.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hadzic, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Harbison, S. A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Harteveld, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hjort, B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hollard, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoff-Olsen, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huels, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Keyser, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maronas, O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
McCallum, N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Moore, D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Morling, N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niederstaetter, H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Noel, F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parson, W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Phillips, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Popielarz, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roeder, A. D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Salvaderi, L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sauer, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schneider, P. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shanthan, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Court, D. SyndercombeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Turanska, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van Oorschot, R. A. H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vennemann, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vidaki, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zatkalikova, L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ballantyne, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-451562
DOI: 10.1016/j.fsigen.2013.09.009
Journal or Publication Title: Forensic Sci. Int.-Genet.
Volume: 8
Number: 1
Page Range: S. 203 - 213
Date: 2014
Publisher: ELSEVIER IRELAND LTD
Place of Publication: CLARE
ISSN: 1878-0326
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BODY-FLUID IDENTIFICATION; POLYMERASE CHAIN-REACTION; MESSENGER-RNA BIOMARKERS; QUANTITATIVE RT-PCR; FORENSIC IDENTIFICATION; SEMEN STAINS; CAPILLARY-ELECTROPHORESIS; SALIVA STAINS; DRIED BLOOD; EXPRESSIONMultiple languages
Genetics & Heredity; Medicine, LegalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/45156

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