Universität zu Köln

Bader, Andreas Matthaeus, Brodarac, Andreja, Klose, Kristin, Bieback, Karen, Choi, Yeong-Hoon, Kang, Kyung-Sun, Kurtz, Andreas ORCID: 0000-0003-3301-6546 and Stamm, Christof (2014). Cord Blood Mesenchymal Stromal Cell-Conditioned Medium Protects Endothelial Cells via STAT3 Signaling. Cell. Physiol. Biochem., 34 (3). S. 646 - 658. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background/Aims: Cell-based therapies may be useful for treating ischemic diseases, but the underlying mechanisms are incompletely understood. We investigated the impact of cord blood mesenchymal stromal cell (CBMSC)- or fibroblast (FB)-secreted factors on starved endothelial cells and determined the relevant intracellular signaling pathways. Methods: HUVECs were subjected to glucose/serum deprivation (GSD) in hypoxia or normoxia, in presence of CBMSC- or FB-conditioned medium (CM). Viability and proliferation were determined via WST-8 conversion and BrdU incorporation. Apoptosis was quantified by annexin V/ethidium homodimer-III staining, nuclear fragmentation and cell morphology. mRNA expression and protein phosphorylation were determined by real-time qPCR and western blot. Experiments were repeated in presence of small molecule inhibitors. Results: The negative impact of GSD was most pronounced at 21% O-2. Here, medium of CBMSCs and FBs increased viability and proliferation and reduced apoptosis of HUVECs. This was associated with increased STAT3 and ERK1/2 phosphorylation and BCL-2 expression. Under STAT3 inhibition, the beneficial effect of CBMSC-CM on viability and BCL-2 expression was abolished. Conclusion: Factors released by CBMSCs protect endothelial cells from the deleterious impact of GSD by activation of the STAT3 survival pathway. However, this phenomenon is not CBMSC-specific and can be reproduced using juvenile fibroblasts. Copyright (C) 2014 S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bader, Andreas Matthaeus UNSPECIFIED UNSPECIFIED UNSPECIFIED Brodarac, Andreja UNSPECIFIED UNSPECIFIED UNSPECIFIED Klose, Kristin UNSPECIFIED UNSPECIFIED UNSPECIFIED Bieback, Karen UNSPECIFIED UNSPECIFIED UNSPECIFIED Choi, Yeong-Hoon UNSPECIFIED UNSPECIFIED UNSPECIFIED Kang, Kyung-Sun UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurtz, Andreas UNSPECIFIED orcid.org/0000-0003-3301-6546 UNSPECIFIED Stamm, Christof UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-451842
DOI:	10.1159/000363030
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	34
Number:	3
Page Range:	S. 646 - 658
Date:	2014
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRITICAL LIMB ISCHEMIA; STEM-CELLS; IN-VITRO; BONE-MARROW; MYOCARDIAL-INFARCTION; PARACRINE MECHANISMS; PROMOTE ANGIOGENESIS; HYPOXIA; APOPTOSIS; TRANSPLANTATION Multiple languages Cell Biology; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/45184