Bognar, Peter, Nemeth, Ilona, Mayer, Balazs ORCID: 0000-0003-3577-3823, Haluszka, Dora, Wikonkal, Norbert, Ostorhazi, Eszter, John, Susan, Paulsson, Mats, Smyth, Neil, Pasztoi, Maria, Buzas, Edit I., Szipocs, Robert, Kolonics, Attila, Temesvari, Erzsebet ORCID: 0000-0002-6403-5478 and Karpati, Sarolta ORCID: 0000-0002-8472-0712 (2014). Reduced Inflammatory Threshold Indicates Skin Barrier Defect in Transglutaminase 3 Knockout Mice. J. Invest. Dermatol., 134 (1). S. 105 - 112. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1523-1747

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Abstract

Recently, a transglutaminase 3 knockout (TGM3/KO) mouse was generated that showed impaired hair development, but no gross defects in the epidermal barrier, although increased fragility of isolated corneocytes was demonstrated. Here we investigated the functionality of skin barrier in vivo by percutaneous sensitization to FITC in TGM3/KO (n=64) and C57BL/6 wild-type (WT) mice (n=36). Cutaneous inflammation was evaluated by mouse ear swelling test (MEST), histology, serum IgE levels, and by flow cytometry from draining lymph nodes. Inflammation-induced significant MEST difference (P < 0.0001) was detected between KO and WT mice and was supported also by histopathology. A significant increase of CD4+ CD25 +-activated T cells (P < 0.01) and elevated serum IgE levels (P < 0.05) in KO mice indicated more the development of FITC sensitization than an irritative reaction. Propionibacter acnes-induced intracutaneous inflammation showed no difference (P=0.2254) between the reactivity of WT and KO immune system. As in vivo tracer, FITC penetration from skin surface followed by two-photon microscopy demonstrated a more invasive percutaneous penetration in KO mice. The clinically uninvolved skin in TGM3/KO mice showed impaired barrier function and higher susceptibility to FITC sensitization indicating that TGM3 has a significant contribution to the functionally intact cutaneous barrier.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Bognar, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nemeth, IlonaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mayer, BalazsUNSPECIFIEDorcid.org/0000-0003-3577-3823UNSPECIFIED
Haluszka, DoraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wikonkal, NorbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ostorhazi, EszterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
John, SusanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Paulsson, MatsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Smyth, NeilUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pasztoi, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Buzas, Edit I.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Szipocs, RobertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kolonics, AttilaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Temesvari, ErzsebetUNSPECIFIEDorcid.org/0000-0002-6403-5478UNSPECIFIED
Karpati, SaroltaUNSPECIFIEDorcid.org/0000-0002-8472-0712UNSPECIFIED
URN: urn:nbn:de:hbz:38-452400
DOI: 10.1038/jid.2013.307
Journal or Publication Title: J. Invest. Dermatol.
Volume: 134
Number: 1
Page Range: S. 105 - 112
Date: 2014
Publisher: ELSEVIER SCIENCE INC
Place of Publication: NEW YORK
ISSN: 1523-1747
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
FLUORESCEIN-ISOTHIOCYANATE; CONTACT HYPERSENSITIVITY; SENSITIZATION PHASE; KERATINOCYTE TRANSGLUTAMINASE; LAMELLAR ICHTHYOSIS; ATOPIC-DERMATITIS; MICROSCOPY; MUTATIONS; CELLS; TGASE-3Multiple languages
DermatologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/45240

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