Universität zu Köln

Ojanpera, Ilkka ORCID: 0000-0001-5790-1473, Sajantila, Antti ORCID: 0000-0003-4117-5248, Vinogradova, Larisa, Thomas, Andreas ORCID: 0000-0003-1199-0743, Schaenzer, Wilhelm and Thevis, Mario (2013). Post-mortem vitreous humour as potential specimen for detection of insulin analogues by LC-MS/MS. Forensic Sci.Int., 233 (1-3). S. 328 - 333. CLARE: ELSEVIER IRELAND LTD. ISSN 1872-6283

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Abstract

Differentiation of insulin analogues is required in forensic and clinical toxicology as well as in sports doping control. Immunoassay results provide only weak evidence for exogenous administration of insulin, as concentrations cannot be reliably interpreted and specific information on the insulin species remains unknown. In post-mortem blood, insulin degrades rapidly. In this study, improved methodology consisting of precipitation of proteins, immunoaffinity purification and liquid chromatography coupled to high resolution/high accuracy mass spectrometry were applied to post-mortem vitreous humour. Ten successive cases with a post-mortem interval from four to ten days were investigated for insulin analogues. The cause of death in these cases was connected with diabetes and its complications, as well as with chronic cardiovascular disease, alcoholism and cancer. In all cases, the manner of death was natural (disease). Insulin was positively detected in post-mortem vitreous humour in three cases out of ten by mass spectrometry. In two cases, the method revealed the long-acting insulin glargine (Lantus) metabolite M2 (DesB31-32 Lantus), and human insulin was detected in one case. The findings were in agreement with the documented history of insulin medication. No other obvious reason could be found for the failure of detecting insulins in the other cases than insulin degradation during the lengthy postmortem interval. Vitreous humour is still a most prospective specimen for detection of insulin analogues post-mortem. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ojanpera, Ilkka UNSPECIFIED orcid.org/0000-0001-5790-1473 UNSPECIFIED Sajantila, Antti UNSPECIFIED orcid.org/0000-0003-4117-5248 UNSPECIFIED Vinogradova, Larisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-470210
DOI:	10.1016/j.forsciint.2013.10.009
Journal or Publication Title:	Forensic Sci.Int.
Volume:	233
Number:	1-3
Page Range:	S. 328 - 333
Date:	2013
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1872-6283
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LIQUID-CHROMATOGRAPHY; IMMUNOAFFINITY PURIFICATION; SYNTHETIC ANALOGS; CROSS-REACTIVITY; GROWTH-HORMONE; HUMAN URINE; ASSAYS Multiple languages Medicine, Legal Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47021