Universität zu Köln

Benstein, Ruben Maximilian ORCID: 0000-0002-5799-7572, Ludewig, Katja, Wulfert, Sabine, Wittek, Sebastian, Gigolashvili, Tamara ORCID: 0000-0002-0416-4796, Frerigmann, Henning ORCID: 0000-0002-7067-2721, Gierth, Markus, Fluegge, Ulf-Ingo and Krueger, Stephan (2013). Arabidopsis Phosphoglycerate Dehydrogenase1 of the Phosphoserine Pathway Is Essential for Development and Required for Ammonium Assimilation and Tryptophan Biosynthesis. Plant Cell, 25 (12). S. 5011 - 5030. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

In plants, two independent serine biosynthetic pathways, the photorespiratory and glycolytic phosphoserine (PS) pathways, have been postulated. Although the photorespiratory pathway is well characterized, little information is available on the function of the PS pathway in plants. Here, we present a detailed characterization of phosphoglycerate dehydrogenases (PGDHs) as components of the PS pathway in Arabidopsis thaliana. All PGDHs localize to plastids and possess similar kinetic properties, but they differ with respect to their sensitivity to serine feedback inhibition. Furthermore, analysis of pgdh1 and phosphoserine phosphatase mutants revealed an embryo-lethal phenotype and PGDH1-silenced lines were inhibited in growth. Metabolic analyses of PGDH1-silenced lines grown under ambient and high CO2 conditions indicate a direct link between PS biosynthesis and ammonium assimilation. In addition, we obtained several lines of evidence for an interconnection between PS and tryptophan biosynthesis, because the expression of PGDH1 and PHOSPHOSERINE AMINOTRANSFERASE1 is regulated by MYB51 and MYB34, two activators of tryptophan biosynthesis. Moreover, the concentration of tryptophan-derived glucosinolates and auxin were reduced in PGDH1-silenced plants. In essence, our results provide evidence for a vital function of PS biosynthesis for plant development and metabolism.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Benstein, Ruben Maximilian UNSPECIFIED orcid.org/0000-0002-5799-7572 UNSPECIFIED Ludewig, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Wulfert, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Wittek, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Gigolashvili, Tamara UNSPECIFIED orcid.org/0000-0002-0416-4796 UNSPECIFIED Frerigmann, Henning UNSPECIFIED orcid.org/0000-0002-7067-2721 UNSPECIFIED Gierth, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Fluegge, Ulf-Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-471615
DOI:	10.1105/tpc.113.118992
Journal or Publication Title:	Plant Cell
Volume:	25
Number:	12
Page Range:	S. 5011 - 5030
Date:	2013
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AMINO-ACIDS; SERINE BIOSYNTHESIS; 3-PHOSPHOGLYCERATE DEHYDROGENASE; ESCHERICHIA-COLI; D-3-PHOSPHOGLYCERATE DEHYDROGENASE; GLUCOSINOLATE BIOSYNTHESIS; AUXIN BIOSYNTHESIS; MERISTEM GROWTH; HIGHER-PLANTS; THALIANA Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47161