Spallek, Thomas ORCID: 0000-0003-4425-1015, Beck, Martina, Ben Khaled, Sara, Salomon, Susanne, Bourdais, Gildas ORCID: 0000-0001-9409-1107, Schellmann, Swen and Robatzek, Silke (2013). ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity. PLoS Genet., 9 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7404

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Abstract

The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and is internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis in Arabidopsis. VPS37-1 co-localizes with FLS2 at endosomes and immunoprecipitates with the receptor upon flg22 elicitation. Vps37-1 mutants are reduced in flg22-induced FLS2 endosomes but not in endosomes labeled by Rab5 GTPases suggesting a defect in FLS2 trafficking rather than formation of endosomes. FLS2 localizes to the lumen of multivesicular bodies, but this is altered in vps37-1 mutants indicating compromised endosomal sorting of FLS2 by ESCRT-I loss-of-function. VPS37-1 and VPS28-2 are critical for immunity against bacterial infection through a role in stomatal closure. Our findings identify that VPS37-1, and likewise VPS28-2, regulate late FLS2 endosomal sorting and reveals that ESCRT-I is critical for flg22-activated stomatal defenses involved in plant immunity.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Spallek, ThomasUNSPECIFIEDorcid.org/0000-0003-4425-1015UNSPECIFIED
Beck, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ben Khaled, SaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Salomon, SusanneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bourdais, GildasUNSPECIFIEDorcid.org/0000-0001-9409-1107UNSPECIFIED
Schellmann, SwenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Robatzek, SilkeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-471629
DOI: 10.1371/journal.pgen.1004035
Journal or Publication Title: PLoS Genet.
Volume: 9
Number: 12
Date: 2013
Publisher: PUBLIC LIBRARY SCIENCE
Place of Publication: SAN FRANCISCO
ISSN: 1553-7404
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GOLGI NETWORK/EARLY ENDOSOME; RECOGNITION RECEPTOR FLS2; PLASMA-MEMBRANE; INNATE IMMUNITY; ABSCISIC-ACID; MULTIVESICULAR BODIES; FLAGELLIN SENSING2; ARABIDOPSIS CELLS; ENDOCYTOSIS; TRAFFICKINGMultiple languages
Genetics & HeredityMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/47162

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