Universität zu Köln

Eduardoff, Mayra, Huber, Gabriela, Bayer, Birgit, Schmid, Dagmar, Anslinger, Katja, Goebel, Tanja, Zimmermann, Bettina, Schneider, Peter M. ORCID: 0000-0003-0744-2349, Roeck, Alexander W. and Parson, Walther ORCID: 0000-0002-5692-2392 (2013). Mass spectrometric base composition profiling: Implications for forensic mtDNA databasing. Forensic Sci. Int.-Genet., 7 (6). S. 587 - 593. CLARE: ELSEVIER IRELAND LTD. ISSN 1878-0326

Full text not available from this repository.

Abstract

In forensic genetics mitochondrial DNA (mtDNA) is usually analyzed by direct Sanger-type sequencing (STS). This method is known to be laborious and sometimes prone to human error. Alternative methods have been proposed that lead to faster results. Among these are methods that involve mass-spectrometry resulting in base composition profiles that are, by definition, less informative than the full nucleotide sequence. Here, we applied a highly automated electrospray ionization mass spectrometry (ESI-MS) system (PLEX-ID) to an mtDNA population study to compare its performance with respect to throughput and concordance to STS. We found that the loss of information power was relatively low compared to the gain in speed and analytical standardization. The detection of point and length heteroplasmy turned out to be roughly comparable between the technologies with some individual differences related to the processes. We confirm that ESI-MS provides a valuable platform for analyzing mtDNA variation that can also be applied in the forensic context. (C) 2013 The Authors. Published by Elsevier Ireland Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eduardoff, Mayra UNSPECIFIED UNSPECIFIED UNSPECIFIED Huber, Gabriela UNSPECIFIED UNSPECIFIED UNSPECIFIED Bayer, Birgit UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmid, Dagmar UNSPECIFIED UNSPECIFIED UNSPECIFIED Anslinger, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Goebel, Tanja UNSPECIFIED UNSPECIFIED UNSPECIFIED Zimmermann, Bettina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Peter M. UNSPECIFIED orcid.org/0000-0003-0744-2349 UNSPECIFIED Roeck, Alexander W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Parson, Walther UNSPECIFIED orcid.org/0000-0002-5692-2392 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-471714
DOI:	10.1016/j.fsigen.2013.05.007
Journal or Publication Title:	Forensic Sci. Int.-Genet.
Volume:	7
Number:	6
Page Range:	S. 587 - 593
Date:	2013
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1878-0326
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CONTROL REGION; MITOCHONDRIAL; IDENTIFICATION; HETEROPLASMY; LENGTH Multiple languages Genetics & Heredity; Medicine, Legal Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47171