Universität zu Köln

Rueschoff, Josef, Kerr, Keith M., Grote, Hans J., Middel, Peter, von Heydebreck, Anja, Alves, Venancio A., Baldus, Stephan E., Buettner, Reinhard, Carvalho, Lina ORCID: 0000-0001-8349-4488, Fink, Ludger, Jochum, Wolfram, Lo, Anthony W. I., Lopez-Rios, Fernando, Marx, Alexander, Molina, Thierry J., Olszewski, Wlodzimierz T., Rieker, Ralf J., Volante, Marco, Thunnissen, Erik ORCID: 0000-0001-5355-8508, Wrba, Fritz, Celik, Ilhan and Stoerkel, Stephan (2013). Reproducibility of Immunohistochemical Scoring for Epidermal Growth Factor Receptor Expression in Non-Small Cell Lung Cancer Round Robin Test. Arch. Pathol. Lab. Med., 137 (9). S. 1255 - 1262. NORTHFIELD: COLL AMER PATHOLOGISTS. ISSN 1543-2165

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Abstract

Context.-The addition of cetuximab to first-line chemotherapy substantially prolonged survival in patients with advanced non-small cell lung cancerwhose tumors expressed high levels of epidermal growth factor receptor (EGFR; immunohistochemistry score of >= 200 on a scale of 0-300). Objective.-To evaluate the interobserver reproducibility of this EGFR immunohistochemistry scoring system, based on both the tumor cell membrane staining intensity (graded 0-3+) and the percentage of cells staining at each intensity. Design.-In parts 1 (initial feasibility study) and 2 of this 2-part round robin test, sections of different non-small cell lung cancer tissue microarrays were stained in a central reference laboratory. Following reference evaluation, EGFR expression in 30 selected tumor cores was characterized in serial sections by lung cancer pathology specialists. The reproducibility of scoring by different raters was assessed. Analysis of between-rater agreement was based on the allocation of EGFR immunohistochemistry scores into low(, <200) and high-(>= 200) EGFR expression groups. Results.-After discussion with raters of the issues impacting reproducibility identified in part 1 and following adjustment of processes, part 2 of the round robin test showed a high interobserver agreement in EGFR immunohistochemistry scoring, with an overall concordance rate of 90.9% and a mean coefficient of 0.812. Specimens with a reference EGFR immunohistochemistry score of lower than 200 and of 200 or higher showed mean concordance rates of 94.7% and 85.6%, respectively. Conclusions.-After appropriate training, assessing EGFR expression by this immunohistochemistry-based method allowed a highly reproducible allocation of non-small cell lung cancers into clinically relevant high-or low-EGFR expression groups.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rueschoff, Josef UNSPECIFIED UNSPECIFIED UNSPECIFIED Kerr, Keith M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Grote, Hans J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Middel, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED von Heydebreck, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Alves, Venancio A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baldus, Stephan E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Buettner, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Carvalho, Lina UNSPECIFIED orcid.org/0000-0001-8349-4488 UNSPECIFIED Fink, Ludger UNSPECIFIED UNSPECIFIED UNSPECIFIED Jochum, Wolfram UNSPECIFIED UNSPECIFIED UNSPECIFIED Lo, Anthony W. I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lopez-Rios, Fernando UNSPECIFIED UNSPECIFIED UNSPECIFIED Marx, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Molina, Thierry J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Olszewski, Wlodzimierz T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rieker, Ralf J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Volante, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Thunnissen, Erik UNSPECIFIED orcid.org/0000-0001-5355-8508 UNSPECIFIED Wrba, Fritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Celik, Ilhan UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoerkel, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-476289
DOI:	10.5858/arpa.2012-0605-OA
Journal or Publication Title:	Arch. Pathol. Lab. Med.
Volume:	137
Number:	9
Page Range:	S. 1255 - 1262
Date:	2013
Publisher:	COLL AMER PATHOLOGISTS
Place of Publication:	NORTHFIELD
ISSN:	1543-2165
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EGFR EXPRESSION; COPY NUMBER; CHEMOTHERAPY; PROTEIN; GENE; CARCINOMAS; BIOMARKERS; CETUXIMAB; HER2 Multiple languages Medical Laboratory Technology; Medicine, Research & Experimental; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47628