Universität zu Köln

Nejadnik, Hossein, Henning, Tobias D., Sutton, Elizabeth J., Baehner, Frederick, Horvai, Andrew, Sennino, Barbara, McDonald, Donald, Meier, Reinhard ORCID: 0000-0002-2169-4780, Misselwitz, Bernd, Link, Thomas M. and Daldrup-Link, Heike E. ORCID: 0000-0002-4929-819X (2012). MR Imaging Features of Gadofluorine-Labeled Matrix-Associated Stem Cell Implants in Cartilage Defects. PLoS One, 7 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Objectives: The purpose of our study was to assess the chondrogenic potential and the MR signal effects of GadofluorineM-Cy labeled matrix associated stem cell implants (MASI) in pig knee specimen. Materials and Methods: Human mesenchymal stem cells (hMSCs) were labeled with the micelle-based contrast agent GadofluorineM-Cy. Ferucarbotran-labeled hMSCs, non-labeled hMSCs and scaffold only served as controls. Chondrogenic differentiation was induced and gene expression and histologic evaluation were performed. The proportions of spindle-shaped vs. round cells of chondrogenic pellets were compared between experimental groups using the Fisher's exact test. Labeled and unlabeled hMSCs and chondrocytes in scaffolds were implanted into cartilage defects of porcine femoral condyles and underwent MR imaging with T1- and T2-weighted SE and GE sequences. Contrast-to-noise ratios (CNR) between implants and adjacent cartilage were determined and analyzed for significant differences between different experimental groups using the Kruskal-Wallis test. Significance was assigned for p<0.017, considering a Bonferroni correction for multiple comparisons. Results: Collagen type II gene expression levels were not significantly different between different groups (p>0.017). However, hMSC differentiation into chondrocytes was superior for unlabeled and GadofluorineM-Cy-labeled cells compared with Ferucarbotran-labeled cells, as evidenced by a significantly higher proportion of spindle cells in chondrogenic pellets (p<0.05). GadofluorineM-Cy-labeled hMSCs and chondrocytes showed a positive signal effect on T1-weighted images and a negative signal effect on T2-weighted images while Ferucarbotran-labeled cells provided a negative signal effect on all sequences. CNR data for both GadofluorineM-Cy-labeled and Ferucarbotran-labeled hMSCs were significantly different compared to unlabeled control cells on T1-weighted SE and T2*-weighted MR images (p<0.017). Conclusion: hMSCs can be labeled by simple incubation with GadofluorineM-Cy. The labeled cells provide significant MR signal effects and less impaired chondrogenesis compared to Ferucarbotran-labeled hMSCs. Thus, GadoflurineM-Cy might represent an alternative MR cell marker to Ferucarbotran, which is not distributed any more in Europe or North America.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nejadnik, Hossein UNSPECIFIED UNSPECIFIED UNSPECIFIED Henning, Tobias D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sutton, Elizabeth J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baehner, Frederick UNSPECIFIED UNSPECIFIED UNSPECIFIED Horvai, Andrew UNSPECIFIED UNSPECIFIED UNSPECIFIED Sennino, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED McDonald, Donald UNSPECIFIED UNSPECIFIED UNSPECIFIED Meier, Reinhard UNSPECIFIED orcid.org/0000-0002-2169-4780 UNSPECIFIED Misselwitz, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Link, Thomas M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Daldrup-Link, Heike E. UNSPECIFIED orcid.org/0000-0002-4929-819X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-476737
DOI:	10.1371/journal.pone.0049971
Journal or Publication Title:	PLoS One
Volume:	7
Number:	12
Date:	2012
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AUTOLOGOUS CHONDROCYTE IMPLANTATION; IN-VIVO; CONTRAST AGENTS; REPAIR; TRANSPLANTATION; KNEE; CHONDROGENESIS; FERUCARBOTRAN; REGENERATION; TRACKING Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47673