Universität zu Köln

Pacioni, Simone, Rueger, Maria Adele, Nistico, Giuseppe, Bornstein, Stefan R., Park, Deric M., McKay, Ron D. and Androutsellis-Theotokis, Andreas (2012). Fast, Potent Pharmacological Expansion of Endogenous Hes3+/Sox2+Cells in the Adult Mouse and Rat Hippocampus. PLoS One, 7 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The adult hippocampus is involved in learning and memory. As a consequence, it is a brain region of remarkable plasticity. This plasticity exhibits itself both as cellular changes and neurogenesis. For neurogenesis to occur, a population of local stem cells and progenitor cells is maintained in the adult brain and these are able to proliferate and differentiate into neurons which contribute to the hippocampal circuitry. There is much interest in understanding the role of immature cells in the hippocampus, in relation to learning and memory. Methods and mechanisms that increase the numbers of these cells will be valuable in this research field. We show here that single injections of soluble factors into the lateral ventricle of adult rats and mice induces the rapid (within one week) increase in the number of putative stem cells/progenitor cells in the hippocampus. The established progenitor marker Sox2 together with the more recently established marker Hes3, were used to quantify the manipulation of the Sox2/Hes3 double-positive cell population. We report that in both adult rodent species, Sox2+/Hes3+ cell numbers can be increased within one week. The most prominent increase was observed in the hilus of the dentate gyrus. This study presents a fast, pharmacological method to manipulate the numbers of endogenous putative stem cells/progenitor cells. This method may be easily modified to alter the degree of activation (e. g. by the use of osmotic pumps for delivery, or by repeat injections through implanted cannulas), in order to be best adapted to different paradigms of research (neurodegenerative disease, neuroprotection, learning, memory, plasticity, etc).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pacioni, Simone UNSPECIFIED UNSPECIFIED UNSPECIFIED Rueger, Maria Adele UNSPECIFIED UNSPECIFIED UNSPECIFIED Nistico, Giuseppe UNSPECIFIED UNSPECIFIED UNSPECIFIED Bornstein, Stefan R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Park, Deric M. UNSPECIFIED UNSPECIFIED UNSPECIFIED McKay, Ron D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Androutsellis-Theotokis, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-476791
DOI:	10.1371/journal.pone.0051630
Journal or Publication Title:	PLoS One
Volume:	7
Number:	12
Date:	2012
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NEURAL STEM-CELLS; CENTRAL-NERVOUS-SYSTEM; NEUROGENESIS IN-VITRO; SUBVENTRICULAR ZONE; GROWTH-FACTOR; PROGENITOR PROLIFERATION; DENTATE GYRUS; MAMMALIAN FOREBRAIN; HUNTINGTONS-DISEASE; GENE-EXPRESSION Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47679