Universität zu Köln

Higgins, Paul G. ORCID: 0000-0001-8677-9454, Janssen, Katharina, Fresen, Maximilian M., Wisplinghoff, Hilmar and Seifert, Harald (2012). Molecular Epidemiology of Acinetobacter baumannii Bloodstream Isolates Obtained in the United States from 1995 to 2004 Using rep-PCR and Multilocus Sequence Typing. J. Clin. Microbiol., 50 (11). S. 3493 - 3501. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-660X

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Abstract

Using a repetitive-sequence-based (rep)-PCR (DiversiLab), we have molecularly typed Acinetobacter nosocomial bloodstream isolates (Acinetobacter baumannii [n = 187], Acinetobacter pittii [n = 23], and Acinetobacter nosocomialis [n = 61]) obtained from patients hospitalized in U. S. hospitals over a 10-year period (1995-2004) during a nationwide surveillance study (Surveillance and Control of Pathogens of Epidemiological Importance [SCOPE]). Patterns of A. baumannii rep-PCR were compared to those of previously identified international clonal lineages (ICs) and were further investigated by multilocus sequence typing (MLST) to compare the two typing methods. Forty-seven of the A. baumannii isolates clustered with the previously defined IC 2. ICs 1, 3, 6, and 7 were also detected. The remaining 81 isolates were unrelated to the described ICs. In contrast, A. pittii and A. nosocomialis isolates were more heterogeneous, as determined by rep-PCR. Our MLST results were in good correlation with the rep-PCR clusters. Our study confirms previous data indicating the predominance of a few major clonal A. baumannii lineages in the United States, particularly IC 2. The presence in the United States of A. baumannii ICs 1, 2, and 3 from as early as 1995 suggests that global dissemination of these lineages was an early event.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Janssen, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Fresen, Maximilian M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wisplinghoff, Hilmar UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-480562
DOI:	10.1128/JCM.01759-12
Journal or Publication Title:	J. Clin. Microbiol.
Volume:	50
Number:	11
Page Range:	S. 3493 - 3501
Date:	2012
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-660X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PREVALENT OXA CARBAPENEMASES; FIELD GEL-ELECTROPHORESIS; POLYMERASE-CHAIN-REACTION; INTERLABORATORY REPRODUCIBILITY; ANTIBIOTIC-RESISTANCE; HOSPITALS; DNA; INFECTIONS; STRAINS; GENES Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48056