Universität zu Köln

von Brandenstein, M., Schlosser, M., Richter, C., Depping, R. and Fries, J. W. U. (2012). ETS-dependent p16(INK4a) and p21(warf1/cip1) gene expression upon endothelin-1 stimulation in malignant versus and non-malignant proximal tubule cells. Life Sci., 91 (13-14). S. 562 - 572. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1879-0631

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Abstract

Aim: Cellular senescence, leading to cell death through prevention of regular cell renewal, is associated with the upregulation of the tumor suppressor gene p16(INK4a). While this mechanism has been described as leading to progressive nephron loss, p16(INK4a) upregulation in renal cell carcinoma has been linked to a diseasespecific improved patient survival rate. While in both conditions endothelin-1 is also upregulated, the signaling pathway connecting ET-1 to p16(INK4a) has not been characterized until this study. Main methods: Cell culture, qRT-PCR, Western Blot, immunoprecipitation (IP), proximity ligation assay (PLA), and non-radioactive electrophoretic mobility shift assay (EMSA). Key findings: In malignant renal proximal tumor cells (Caki-1), an activation of p16(INK4a) and p21(waf1/cip1) was observed. An increased expression of E-26 transformation-specific (ETS) transcription factors was detectable. Using specific antibodies, a complex formation between ETS1 and extracellular signal-regulated kinase-2 (ERK2) was shown. A further complex partner was Mxi2. EMSA with supershift analysis for ETS1 and Mxi2 indicated the involvement of both factors in the protein-DNA interaction. After specifically blocking the endothelin receptors, ETS1 expression was significantly downregulated. However, the endothelin B receptor dependent downregulation was stronger than that of the A receptor. In contrast, primary proximal tubule cells showed a nuclear decrease after ET-1 stimulation. This indicates that other ETS members may be involved in the observed p16(INK4a) upregulation (as described in the literature). Significance: ETS1, ERK2 and Mxi2 are important complex partners initiating increased p16(INK4a) and p21w(af1/cip1) activation in renal tumor cells. (c) 2012 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code von Brandenstein, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlosser, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Richter, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Depping, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, J. W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481019
DOI:	10.1016/j.lfs.2012.04.014
Journal or Publication Title:	Life Sci.
Volume:	91
Number:	13-14
Page Range:	S. 562 - 572
Date:	2012
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1879-0631
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIVATED PROTEIN-KINASE; SMOOTH-MUSCLE-CELLS; TRANSCRIPTION FACTORS; NUCLEAR TRANSLOCATION; GLOMERULAR-DISEASE; CARCINOMA-CELLS; MAP KINASE; SENESCENCE; GROWTH; MXI2 Multiple languages Medicine, Research & Experimental; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48101