Universität zu Köln

Thomas, Andreas ORCID: 0000-0003-1199-0743, Walpurgis, Katja, Krug, Oliver, Schaenzer, Wilhelm and Thevis, Mario (2012). Determination of prohibited, small peptides in urine for sports drug testing by means of nano-liquid chromatography/benchtop quadrupole orbitrap tandem-mass spectrometry. J. Chromatogr. A, 1259. S. 251 - 258. AMSTERDAM: ELSEVIER. ISSN 1873-3778

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Abstract

In the present study, a screening assay was developed comprising 11 prohibited peptides (<1.5 kDa) that are sufficiently purified from urine using weak cation exchange with subsequent determination of all substances by means of nanoUHPLC separation coupled to high resolution tandem mass spectrometry. These peptides included Gonadorelin (LH-RH), Desmopressin and 9 growth hormone releasing peptides (GHRP-1. -2, -4, -5, -6. Hexarelin. Alexamorelin, Ipamorelin and a GHRP-2 metabolite); however, the procedure is expandable to further target analytes or metabolites. The method was validated with a main focus on qualitative result interpretation considering the parameters specificity, linearity (0-500 pg/mL), recovery (45-95%). precision (<20% at 100 pg/mL). limits of detection (2-10 pg/mL), robustnesss and ion suppression. The proof-of-principle was shown by analysing excretion study urine samples for LHRH, Desmopressin and GHRP-2. (C) 2012 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Walpurgis, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Krug, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481034
DOI:	10.1016/j.chroma.2012.07.022
Journal or Publication Title:	J. Chromatogr. A
Volume:	1259
Page Range:	S. 251 - 258
Date:	2012
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1873-3778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HORMONE; IDENTIFICATION; GHRP-2; VASOPRESSIN; PROTEINS Multiple languages Biochemical Research Methods; Chemistry, Analytical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48103