Universität zu Köln

Nicolas, Armel, Jolinon, Nelly, Alazard-Dany, Nathalie, Barateau, Veronique, Epstein, Alberto L., Greco, Anna, Buening, Hildegard and Salvetti, Anna ORCID: 0000-0003-2007-8350 (2012). Factors influencing helper-independent adeno-associated virus replication. Virology, 432 (1). S. 1 - 10. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 0042-6822

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Abstract

The inability of Adeno-Associated Virus (AAV) to replicate on its own is a strong argument in favor of the use of recombinant AAV vectors for in vivo gene transfer. However, some previous studies suggested that AAV may become replication competent in cells exposed to a genotoxic stress even in the absence of co-infection with a helper virus. To comprehensively explore this phenomenon, we examined AAV genome replication in several human cell lines exposed to different genotoxic conditions. We found that all treatments induced only negligible levels of AAV replication never exceeding ten fold above background. Further investigation indicated that induction of helper-independent AAV replication relied on the synergistic contribution of several extrinsic factors linked to the origin of the cell line and the quality of the AAV preparation. These results further support the notion that helper independent AAV replication cannot occur at significant levels in vivo. (C) 2012 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nicolas, Armel UNSPECIFIED UNSPECIFIED UNSPECIFIED Jolinon, Nelly UNSPECIFIED UNSPECIFIED UNSPECIFIED Alazard-Dany, Nathalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Barateau, Veronique UNSPECIFIED UNSPECIFIED UNSPECIFIED Epstein, Alberto L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Greco, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Buening, Hildegard UNSPECIFIED UNSPECIFIED UNSPECIFIED Salvetti, Anna UNSPECIFIED orcid.org/0000-0003-2007-8350 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481060
DOI:	10.1016/j.virol.2012.05.027
Journal or Publication Title:	Virology
Volume:	432
Number:	1
Page Range:	S. 1 - 10
Date:	2012
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	0042-6822
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADENO-ASSOCIATED VIRUS; HUMAN TISSUES; NONDIVIDING CELLS; DNA-SYNTHESIS; TRANSDUCTION; VECTORS; EXPRESSION; PROTEINS; GENOMES; TRANSCRIPTION Multiple languages Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48106