Universität zu Köln

Karow, Marisa ORCID: 0000-0003-4935-1365, Sanchez, Rodrigo, Schichor, Christian, Masserdotti, Giacomo, Ortega, Felipe ORCID: 0000-0001-6398-9520, Heinrich, Christophe, Gascon, Sergio ORCID: 0000-0001-8756-7082, Khan, Muhammad A., Lie, D. Chichung, Dellavalle, Arianna, Cossu, Giulio ORCID: 0000-0001-5863-9593, Goldbrunner, Roland, Goetz, Magdalena and Berninger, Benedikt ORCID: 0000-0003-2652-2782 (2012). Reprogramming of Pericyte-Derived Cells of the Adult Human Brain into Induced Neuronal Cells. Cell Stem Cell, 11 (4). S. 471 - 477. CAMBRIDGE: CELL PRESS. ISSN 1934-5909

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Abstract

Reprogramming of somatic cells into neurons provides a new approach toward cell-based therapy of neurodegenerative diseases. A major challenge for the translation of neuronal reprogramming into therapy is whether the adult human brain contains cell populations amenable to direct somatic cell conversion. Here we show that cells from the adult human cerebral cortex expressing pericyte hallmarks can be reprogrammed into neuronal cells by retrovirus-mediated coexpression of the transcription factors Sox2 and Mash1. These induced neuronal cells acquire the ability of repetitive action potential firing and serve as synaptic targets for other neurons, indicating their capability of integrating into neural networks. Genetic fate-mapping in mice expressing an inducible Cre recombinase under the tissue-nonspecific alkaline phosphatase promoter corroborated the pericytic origin of the reprogrammed cells. Our results raise the possibility of functional conversion of endogenous cells in the adult human brain to induced neuronal fates.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Karow, Marisa UNSPECIFIED orcid.org/0000-0003-4935-1365 UNSPECIFIED Sanchez, Rodrigo UNSPECIFIED UNSPECIFIED UNSPECIFIED Schichor, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Masserdotti, Giacomo UNSPECIFIED UNSPECIFIED UNSPECIFIED Ortega, Felipe UNSPECIFIED orcid.org/0000-0001-6398-9520 UNSPECIFIED Heinrich, Christophe UNSPECIFIED UNSPECIFIED UNSPECIFIED Gascon, Sergio UNSPECIFIED orcid.org/0000-0001-8756-7082 UNSPECIFIED Khan, Muhammad A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lie, D. Chichung UNSPECIFIED UNSPECIFIED UNSPECIFIED Dellavalle, Arianna UNSPECIFIED UNSPECIFIED UNSPECIFIED Cossu, Giulio UNSPECIFIED orcid.org/0000-0001-5863-9593 UNSPECIFIED Goldbrunner, Roland UNSPECIFIED UNSPECIFIED UNSPECIFIED Goetz, Magdalena UNSPECIFIED UNSPECIFIED UNSPECIFIED Berninger, Benedikt UNSPECIFIED orcid.org/0000-0003-2652-2782 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481158
DOI:	10.1016/j.stem.2012.07.007
Journal or Publication Title:	Cell Stem Cell
Volume:	11
Number:	4
Page Range:	S. 471 - 477
Date:	2012
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1934-5909
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN FIBROBLASTS; DOPAMINERGIC-NEURONS; FUNCTIONAL-NEURONS; POSTNATAL ASTROGLIA; DIRECT CONVERSION; MOUSE; GENERATION; ORIGIN Multiple languages Cell & Tissue Engineering; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48115