Universität zu Köln

Sprinkart, A. M., Block, W., Traeber, F., Meyer, R., Paulin, D., Clemen, C. S., Schroeder, R., Gieseke, J., Schild, H. and Thomas, D. (2012). Characterization of the failing murine heart in a desmin knock-out model using a clinical 3 T MRI scanner. Int. J. Cardiovasc. Imaging, 28 (7). S. 1699 - 1706. DORDRECHT: SPRINGER. ISSN 1573-0743

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Abstract

The purpose of this study was to establish an MRI protocol on a clinical scanner for assessment of left (LV) and right (RV) ventricular myocardial function of the murine heart, and to apply this protocol for the first in vivo assessment of myocardial function in a mouse model of cardiomyopathy (Desmin-/-). MRI was performed on a clinical 3 T whole body MRI system using a dedicated solenoid receive-only coil. Contiguous short axis slices were acquired covering the entire heart using a spoiled cine gradient echo sequence (TR 9-12 ms, TE 3-4 ms, alpha 25A degrees, 1.0 x 0.23 x 0.23 mmA(3)). Global LV- and RV-myocardial functional parameters such as end-diastolic ventricular volume, ejection fraction (EF), LV mass and cardiac output (CO) of Desmin-/- mice and age-matched controls were determined. Global myocardial functional data of healthy controls (n = 4) were in very good agreement with previously reported data. The transgenic mice (n = 8) revealed a significantly reduced LV- and RV-EF as well as CO. Body weight-normalized LV- and RV-end-diastolic volumes and LV mass were significantly increased. In addition desmin deficient mice exhibited segmental wall thinning and akinesia, suggesting myocardial necrosis. This study demonstrates that clinical 3 T MRI-systems may reliably be used for non-invasive assessment of LV- and RV-myocardial function in normal and in genetically engineered mice with cardiomyopathies. In addition, this proof of principle study presents first in vivo MRI data of the cardiac phenotype of desmin knock-out mice.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sprinkart, A. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Block, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Traeber, F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Paulin, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Clemen, C. S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeder, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gieseke, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schild, H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481462
DOI:	10.1007/s10554-011-9990-3
Journal or Publication Title:	Int. J. Cardiovasc. Imaging
Volume:	28
Number:	7
Page Range:	S. 1699 - 1706
Date:	2012
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-0743
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICE LACKING DESMIN; IN-VIVO ASSESSMENT; CARDIAC-FUNCTION; CARDIOMYOPATHY; SKELETAL; MYOPATHY; FAILURE; MUSCLE; CINE; MASS Multiple languages Cardiac & Cardiovascular Systems; Radiology, Nuclear Medicine & Medical Imaging Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48146