Miller, Sharon E., Sahlender, Daniela A., Graham, Stephen C., Hoening, Stefan, Robinson, Margaret S., Peden, Andrew A. and Owen, David J. (2011). The Molecular Basis for the Endocytosis of Small R-SNAREs by the Clathrin Adaptor CALM. Cell, 147 (5). S. 1118 - 1132. CAMBRIDGE: CELL PRESS. ISSN 0092-8674
Full text not available from this repository.Abstract
SNAREs provide a large part of the specificity and energy needed for membrane fusion and, to do so, must be localized to their correct membranes. Here, we show that the R-SNAREs VAMP8, VAMP3, and VAMP2, which cycle between the plasma membrane and endosomes, bind directly to the ubiquitously expressed, PtdIns4, 5P(2)-binding, endocytic clathrin adaptor CALM/PICALM. X-ray crystallography shows that the N-terminal halves of their SNARE motifs bind the CALM(ANTH) domain as helices in a manner that mimics SNARE complex formation. Mutation of residues in the CALM: SNARE interface inhibits binding in vitro and prevents R-SNARE endocytosis in vivo. Thus, CALM: R-SNARE interactions ensure that R-SNAREs, required for the fusion of endocytic clathrin-coated vesicles with endosomes and also for subsequent postendosomal trafficking, are sorted into endocytic vesicles. CALM's role in directing the endocytosis of small R-SNAREs may provide insight into the association of CALM/PICALMmutations with growth retardation, cognitive defects, and Alzheimer's disease.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-485153 | ||||||||||||||||||||||||||||||||
DOI: | 10.1016/j.cell.2011.10.038 | ||||||||||||||||||||||||||||||||
Journal or Publication Title: | Cell | ||||||||||||||||||||||||||||||||
Volume: | 147 | ||||||||||||||||||||||||||||||||
Number: | 5 | ||||||||||||||||||||||||||||||||
Page Range: | S. 1118 - 1132 | ||||||||||||||||||||||||||||||||
Date: | 2011 | ||||||||||||||||||||||||||||||||
Publisher: | CELL PRESS | ||||||||||||||||||||||||||||||||
Place of Publication: | CAMBRIDGE | ||||||||||||||||||||||||||||||||
ISSN: | 0092-8674 | ||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/48515 |
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