Universität zu Köln

Koppen, Tim, Weckmann, Andreas, Mueller, Stefan, Staubach, Simon, Bloch, Wilhelm, Dohmen, R. Juergen and Schwientek, Tilo (2011). Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. Proteomics, 11 (22). S. 4397 - 4411. HOBOKEN: WILEY. ISSN 1615-9861

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Abstract

Distinct types of vesicles are formed in eukaryotic cells that conduct a variable set of functions depending on their origin. One subtype designated circulating microvesicles (MVs) provides a novel form of intercellular communication and recent work suggested the release and uptake of morphogens in vesicles by Drosophila cells. In this study, we have examined cells of the hemocyte-like cell lines Kc167 and S2 and identified secreted vesicles in the culture supernatant. The vesicles were isolated and found to have characteristics comparable to exosomes and plasma membrane MVs released by mammalian cells. In wingless-transfected cells, the full-length protein was detected in the vesicle isolates. Proteomics analyses of the vesicles identified 269 proteins that include various orthologs of marker proteins and proteins with putative functions in vesicle formation and release. Analogous to their mammalian counterparts, the subcellular origin of the vesicular constituents of both cell lines is dominated by membrane-associated and cytosolic proteins with functions that are consistent with their localization in MVs. The analyses revealed a significant overlap of the Kc167 and S2 vesicle proteomes and confirmed a close correlation with non-mammalian and mammalian exosomes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koppen, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Weckmann, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Staubach, Simon UNSPECIFIED UNSPECIFIED UNSPECIFIED Bloch, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Dohmen, R. Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwientek, Tilo UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-485944
DOI:	10.1002/pmic.201000774
Journal or Publication Title:	Proteomics
Volume:	11
Number:	22
Page Range:	S. 4397 - 4411
Date:	2011
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1615-9861
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIVATED PLATELETS; WNT PROTEINS; IN-VITRO; EXOSOMES; SECRETION; RECEPTOR; VESICLES; SIGNALS; INTERNALIZATION; HEDGEHOG Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48594