Universität zu Köln

Sillo, Alessio, Matthias, Jan, Konertz, Roman ORCID: 0000-0002-6488-1885, Bozzaro, Salvatore and Eichinger, Ludwig ORCID: 0000-0003-1594-6117 (2011). Salmonella typhimurium is pathogenic for Dictyostelium cells and subverts the starvation response. Cell Microbiol., 13 (11). S. 1793 - 1812. HOBOKEN: WILEY. ISSN 1462-5822

Full text not available from this repository.

Abstract

In unicellular amoebae, such as Dictyostelium discoideum, bacterial phagocytosis is a food hunting device, while in higher organisms it is the first defence barrier against microbial infection. In both cases, pathogenic bacteria exploit phagocytosis to enter the cell and multiply intracellularly. Salmonella typhimurium, the agent of food-borne gastroenteritis, is phagocytosed by both macrophages and Dictyostelium cells. By using cell biological assays and global transcriptional analysis with DNA microarrays covering the Dictyostelium genome, we show here that S. typhimurium is pathogenic for Dictyostelium cells. Depending on the degree of virulence, which in turn depended on bacterial growth conditions, Salmonella could kill Dictyostelium cells or inhibit their growth and development. In the early phase of infection in non-nutrient buffer, the ingested bacteria escaped degradation, induced a starvation-like transcriptional response but inhibited selectively genes required for chemotaxis and aggregation. This way differentiation of the host cells into spore and stalk cells was blocked or delayed, which in turn is likely to be favourable for the establishment of a replicative niche for Salmonella. Inhibition of the aggregation competence and chemotactic streaming of aggregation-competent cells in the presence of Salmonella suggests interference with cAMP signalling.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sillo, Alessio UNSPECIFIED UNSPECIFIED UNSPECIFIED Matthias, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Konertz, Roman UNSPECIFIED orcid.org/0000-0002-6488-1885 UNSPECIFIED Bozzaro, Salvatore UNSPECIFIED UNSPECIFIED UNSPECIFIED Eichinger, Ludwig UNSPECIFIED orcid.org/0000-0003-1594-6117 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-486011
DOI:	10.1111/j.1462-5822.2011.01662.x
Journal or Publication Title:	Cell Microbiol.
Volume:	13
Number:	11
Page Range:	S. 1793 - 1812
Date:	2011
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1462-5822
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language III SECRETION SYSTEM; CYCLIC-AMP; LEGIONELLA-PNEUMOPHILA; ACTIN CYTOSKELETON; SIGNALING PATHWAYS; ADENYLYL-CYCLASE; EPITHELIAL-CELLS; CLONING VECTORS; DISCOIDEUM; GENE Multiple languages Cell Biology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48601