Universität zu Köln

Franz, Andre, Orth, Michael, Pirson, Paul A., Sonneville, Remi, Blow, J. Julian ORCID: 0000-0002-9524-5849, Gartner, Anton ORCID: 0000-0003-4639-9902, Stemmann, Olaf ORCID: 0000-0003-3044-2515 and Hoppe, Thorsten ORCID: 0000-0002-4734-9352 (2011). CDC-48/p97 Coordinates CDT-1 Degradation with GINS Chromatin Dissociation to Ensure Faithful DNA Replication. Mol. Cell, 44 (1). S. 85 - 97. CAMBRIDGE: CELL PRESS. ISSN 1097-4164

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Abstract

Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. In the licensing step of DNA replication, CDT-1 is loaded onto chromatin to subsequently promote the recruitment of additional replication factors, including CDC-45 and GINS. During the elongation step, the CDC-45/GINS complex moves with the replication fork; however, it is largely unknown how its chromatin association is regulated. Here, we show that the chaperone-like ATPase CDC-48/p97 coordinates degradation of CDT-1 with release of the CDC-45/GINS complex. C. elegans embryos lacking CDC-48 or its cofactors UFD-1/NPL-4 accumulate CDT-1 on mitotic chromatin, indicating a critical role of CDC-48 in CDT-1 turnover. Strikingly, CDC-48(UFD-1/NPL-4)-deficient embryos show persistent chromatin association of CDC-45/GINS, which is a consequence of CDT-1 stabilization. Moreover, our data confirmed a similar regulation in Xenopus egg extracts, emphasizing a conserved coordination of licensing and elongation events during eukaryotic DNA replication by CDC-48/p97.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Franz, Andre UNSPECIFIED UNSPECIFIED UNSPECIFIED Orth, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Pirson, Paul A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sonneville, Remi UNSPECIFIED UNSPECIFIED UNSPECIFIED Blow, J. Julian UNSPECIFIED orcid.org/0000-0002-9524-5849 UNSPECIFIED Gartner, Anton UNSPECIFIED orcid.org/0000-0003-4639-9902 UNSPECIFIED Stemmann, Olaf UNSPECIFIED orcid.org/0000-0003-3044-2515 UNSPECIFIED Hoppe, Thorsten UNSPECIFIED orcid.org/0000-0002-4734-9352 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-487437
DOI:	10.1016/j.molcel.2011.08.028
Journal or Publication Title:	Mol. Cell
Volume:	44
Number:	1
Page Range:	S. 85 - 97
Date:	2011
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1097-4164
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language XENOPUS EGG EXTRACTS; UBIQUITIN-SELECTIVE CHAPERONE; AAA ATPASE CDC48/P97; CAENORHABDITIS-ELEGANS; CELL-CYCLE; C-ELEGANS; S-PHASE; PROTEIN-DEGRADATION; GENOME STABILITY; CDC45 Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48743