Universität zu Köln

Dircksen, Heinrich ORCID: 0000-0001-7815-4868, Neupert, Susanne, Predel, Reinhard, Verleyen, Peter, Huybrechts, Jurgen, Strauss, Johannes, Hauser, Frank, Stafflinger, Elisabeth, Schneider, Martina, Pauwels, Kevin, Schoofs, Liliane ORCID: 0000-0002-1673-432X and Grimmelikhuijzen, Cornelis J. P. (2011). Genomics, Transcriptomics, and Peptidomics of Daphnia pulex Neuropeptides and Protein Hormones. J. Proteome Res., 10 (10). S. 4478 - 4505. WASHINGTON: AMER CHEMICAL SOC. ISSN 1535-3907

Full text not available from this repository.

Abstract

We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerolcinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met(4)-proctolin, short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, and three tachykinins. There are two genes for a preprohormone containing orcomyotropin-like peptides and orcokinins, two genes for N-terminally elongated ITPs, two genes (clustered) for eclosion hormones, two genes (clustered) for bursicons alpha, beta, and two genes (clustered) for glycoproteins GPA2, GPBS, three genes for different allatostatins-C (two of them clustered) and three genes for IGF-related peptides. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer related to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dircksen, Heinrich UNSPECIFIED orcid.org/0000-0001-7815-4868 UNSPECIFIED Neupert, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Verleyen, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Huybrechts, Jurgen UNSPECIFIED UNSPECIFIED UNSPECIFIED Strauss, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Hauser, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Stafflinger, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Pauwels, Kevin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schoofs, Liliane UNSPECIFIED orcid.org/0000-0002-1673-432X UNSPECIFIED Grimmelikhuijzen, Cornelis J. P. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-488542
DOI:	10.1021/pr200284e
Journal or Publication Title:	J. Proteome Res.
Volume:	10
Number:	10
Page Range:	S. 4478 - 4505
Date:	2011
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1535-3907
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRUSTACEAN-HYPERGLYCEMIC-HORMONE; PIGMENT-CONCENTRATING HORMONE; MANDUCA-SEXTA ALLATOTROPIN; RED FLOUR BEETLE; MASS-SPECTROMETRIC CHARACTERIZATION; CRAYFISH PROCAMBARUS-CLARKII; TACHYKININ-RELATED PEPTIDES; PRECURSOR-RELATED PEPTIDES; ALLATOSTATIN-LIKE PEPTIDE; NERVOUS-SYSTEM Multiple languages Biochemical Research Methods Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48854