Pesch, Martina and Huelskamp, Martin (2011). Role of TRIPTYCHON in trichome patterning in Arabidopsis. BMC Plant Biol., 11. LONDON: BMC. ISSN 1471-2229
Full text not available from this repository.Abstract
Background: Trichome patterning in Arabidopsis thaliana is governed by three types of activators, R2R3MYB, bHLH and WD40 proteins, and six R3MYB inhibitors. Among the inhibitors TRIPTYCHON (TRY) seems to fulfill a special function. Its corresponding mutants produce trichome clusters whereas all other inhibitors are involved in trichome density regulation. Results: To better understand the role of TRY in trichome patterning we analyzed its transcriptional regulation. A promoter analysis identified the relevant regulatory region for trichome patterning. This essential region contains a fragment required for a double negative feedback loop such that it mediates the repression of TRY/CPC auto-repression. By transforming single cells of pTRY:GUS lines with p35S:GL1, p35S:GL3 and p35S:TTG1 in the presence or absence of p35S:TRY or p35S:CPC we demonstrate that TRY and CPC can suppress the TRY expression without the transcriptional down regulation of the activators. We further show by promoter/CDS swapping experiments for the R3MYB inhibitors TRY and CPC that the TRY protein has specific properties relevant in the context of both, cluster formation and trichome density. Conclusions: Our identification of a TRY promoter fragment mediating a double negative feedback loop reveals new insight in the regulatory network of the trichome patterning machinery. In addition we show that the auto-repression by TRY can occur without a transcriptional down regulation of the activators, suggesting that the differential complex formation model has a biological significance. Finally we show that the unique role of TRY among the inhibitors is a property of the TRY protein.
Item Type: | Journal Article | ||||||||||||
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URN: | urn:nbn:de:hbz:38-488845 | ||||||||||||
DOI: | 10.1186/1471-2229-11-130 | ||||||||||||
Journal or Publication Title: | BMC Plant Biol. | ||||||||||||
Volume: | 11 | ||||||||||||
Date: | 2011 | ||||||||||||
Publisher: | BMC | ||||||||||||
Place of Publication: | LONDON | ||||||||||||
ISSN: | 1471-2229 | ||||||||||||
Language: | English | ||||||||||||
Faculty: | Unspecified | ||||||||||||
Divisions: | Unspecified | ||||||||||||
Subjects: | no entry | ||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/48884 |
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