Universität zu Köln

Specht, Christian G., Gruenewald, Nora, Pascual, Olivier ORCID: 0000-0002-5730-9880, Rostgaard, Nina, Schwarz, Guenter ORCID: 0000-0002-2118-9338 and Triller, Antoine (2011). Regulation of glycine receptor diffusion properties and gephyrin interactions by protein kinase C. Embo J., 30 (18). S. 3842 - 3854. NEW YORK: NATURE PUBLISHING GROUP. ISSN 0261-4189

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Abstract

Glycine receptors (GlyRs) can dynamically exchange between synaptic and extrasynaptic locations through lateral diffusion within the plasma membrane. Their accumulation at inhibitory synapses depends on the interaction of the beta-subunit of the GlyR with the synaptic scaffold protein gephyrin. An alteration of receptor-gephyrin binding could thus shift the equilibrium between synaptic and extrasynaptic GlyRs and modulate the strength of inhibitory neurotransmission. Using a combination of dynamic imaging and biochemical approaches, we have characterised the molecular mechanism that links the GlyR-gephyrin interaction with GlyR diffusion and synaptic localisation. We have identified a protein kinase C (PKC) phosphorylation site within the cytoplasmic domain of the beta-subunit of the GlyR (residue S403) that causes a reduction of the binding affinity between the receptor and gephyrin. In consequence, the receptor's diffusion in the plasma membrane is accelerated and GlyRs accumulate less strongly at synapses. We propose that the regulation of GlyR dynamics by PKC thus contributes to the plasticity of inhibitory synapses and may be involved in maladaptive forms of synaptic plasticity. The EMBO Journal (2011) 30, 3842-3853. doi: 10.1038/emboj.2011.276; Published online 9 August 2011

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Specht, Christian G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruenewald, Nora UNSPECIFIED UNSPECIFIED UNSPECIFIED Pascual, Olivier UNSPECIFIED orcid.org/0000-0002-5730-9880 UNSPECIFIED Rostgaard, Nina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Guenter UNSPECIFIED orcid.org/0000-0002-2118-9338 UNSPECIFIED Triller, Antoine UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-489033
DOI:	10.1038/emboj.2011.276
Journal or Publication Title:	Embo J.
Volume:	30
Number:	18
Page Range:	S. 3842 - 3854
Date:	2011
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	NEW YORK
ISSN:	0261-4189
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LATERAL DIFFUSION; BETA-SUBUNIT; PHOSPHORYLATION; NEURONS; MEMBRANE; MODULATION; SYNAPSES; DYNAMICS; CHANNELS; EXPRESSION Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48903