Kasper, Hans-Udo, Konze, Elisabeth, Canova, Nikolina Kutinova, Dienes, Hans Peter and Dries, Volker (2011). Cryopreservation of precision cut tissue slices (PCTS): Investigation of morphology and reactivity. Exp. Toxicol. Pathol., 63 (6). S. 575 - 581. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG. ISSN 0940-2993

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Abstract

Precision cut tissue slices (PCTS) represent a suitable and convenient tool for pharmacological, toxicological and morphological studies. Cryopreservation would enable to overcome the shortage of liver tissue, in particular in settings using human liver tissue. We investigated the potential of cryopreservation of porcine PCTS as a morphological tool by rapid freezing with 10% and 30% dimethyl sulfoxide as cryopreservation agents and with or without medium using a Brendel/Vitron tissue slicer. Incubation after thawing was done in a static incubation system. Slices were cultured for 3 h, 6 h, 24 h and 48 h and assessed histologically and immunohistologically for proliferation (Ki67) and spontaneous as well as induced apoptotic activity (M30Cytodeath). Vitality was tested using the Tox-8 test. After cryopreservation, morphology of PCTS was well preserved up to 24 h. A reduction of vitality rate took place. Compared to non-frozen PCTS, the rate of spontaneous proliferation of Kupffer cells and apoptosis of hepatocytes were significantly reduced independent of the freezing conditions. The reactivity of PCTS to apoptotic stimuli was significantly reduced in tissue slices after cryopreservation. Apoptotic stimuli could not induce the same amount of cell deaths compared to non-frozen sections. Thus, cryopreservation of PCTS does interfere with pathomechanisms of apoptosis in PCTS. (C) 2010 Elsevier GmbH. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kasper, Hans-UdoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Konze, ElisabethUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Canova, Nikolina KutinovaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dienes, Hans PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dries, VolkerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-489713
DOI: 10.1016/j.etp.2010.04.010
Journal or Publication Title: Exp. Toxicol. Pathol.
Volume: 63
Number: 6
Page Range: S. 575 - 581
Date: 2011
Publisher: ELSEVIER GMBH, URBAN & FISCHER VERLAG
Place of Publication: JENA
ISSN: 0940-2993
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
RAT-LIVER SLICES; HUMAN HEPATOCYTES; 5 BENZODIAZEPINES; ENZYME-INDUCTION; DRUG-METABOLISM; PHASE-I; CYTOCHROME-P450; TRANSPLANTATION; TOOL; INCUBATIONMultiple languages
Pathology; ToxicologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/48971

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