Universität zu Köln

Coras, Roland, Siebzehnrubl, Florian A., Pauli, Elisabeth, Huttner, Hagen B., Njunting, Marleisje, Kobow, Katja, Villmann, Carmen, Hahnen, Eric, Neuhuber, Winfried, Weigel, Daniel, Buchfelder, Michael, Stefan, Hermann, Beck, Heinz, Steindler, Dennis A. and Bluemcke, Ingmar (2010). Low proliferation and differentiation capacities of adult hippocampal stem cells correlate with memory dysfunction in humans. Brain, 133. S. 3359 - 3373. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2156

Full text not available from this repository.

Abstract

The hippocampal dentate gyrus maintains its capacity to generate new neurons throughout life. In animal models, hippocampal neurogenesis is increased by cognitive tasks, and experimental ablation of neurogenesis disrupts specific modalities of learning and memory. In humans, the impact of neurogenesis on cognition remains unclear. Here, we assessed the neurogenic potential in the human hippocampal dentate gyrus by isolating adult human neural stem cells from 23 surgical en bloc hippocampus resections. After proliferation of the progenitor cell pool in vitro we identified two distinct patterns. Adult human neural stem cells with a high proliferation capacity were obtained in 11 patients. Most of the cells in the high proliferation capacity cultures were capable of neuronal differentiation (53 +/- 13% of in vitro cell population). A low proliferation capacity was observed in 12 specimens, and only few cells differentiated into neurons (4 +/- 2%). This was reflected by reduced numbers of proliferating cells in vivo as well as granule cells immunoreactive for doublecortin, brain-derived neurotrophic factor and cyclin-dependent kinase 5 in the low proliferation capacity group. High and low proliferation capacity groups differed dramatically in declarative memory tasks. Patients with high proliferation capacity stem cells had a normal memory performance prior to epilepsy surgery, while patients with low proliferation capacity stem cells showed severe learning and memory impairment. Histopathological examination revealed a highly significant correlation between granule cell loss in the dentate gyrus and the same patient's regenerative capacity in vitro (r = 0.813; P < 0.001; linear regression: R-adjusted(2) = 0.635), as well as the same patient's ability to store and recall new memories (r = 0.966; P = 0.001; linear regression: R-adjusted(2) = 0.9). Our results suggest that encoding new memories is related to the regenerative capacity of the hippocampus in the human brain.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Coras, Roland UNSPECIFIED UNSPECIFIED UNSPECIFIED Siebzehnrubl, Florian A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pauli, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Huttner, Hagen B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Njunting, Marleisje UNSPECIFIED UNSPECIFIED UNSPECIFIED Kobow, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Villmann, Carmen UNSPECIFIED UNSPECIFIED UNSPECIFIED Hahnen, Eric UNSPECIFIED UNSPECIFIED UNSPECIFIED Neuhuber, Winfried UNSPECIFIED UNSPECIFIED UNSPECIFIED Weigel, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Buchfelder, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Stefan, Hermann UNSPECIFIED UNSPECIFIED UNSPECIFIED Beck, Heinz UNSPECIFIED UNSPECIFIED UNSPECIFIED Steindler, Dennis A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bluemcke, Ingmar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-493025
DOI:	10.1093/brain/awq215
Journal or Publication Title:	Brain
Volume:	133
Page Range:	S. 3359 - 3373
Date:	2010
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2156
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TEMPORAL-LOBE EPILEPSY; DENTATE GYRUS; NEURONAL DIFFERENTIATION; NEUROTROPHIC FACTOR; PATTERN SEPARATION; GRANULE CELLS; NEURAL STEM; NEUROGENESIS; MATURATION; CA3 Multiple languages Clinical Neurology; Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49302