Universität zu Köln

Lee, Jin-Yul, Keep, Richard F., He, Yangdong, Sagher, Oren, Hua, Ya and Xi, Guohua (2010). Hemoglobin and iron handling in brain after subarachnoid hemorrhage and the effect of deferoxamine on early brain injury. J. Cereb. Blood Flow Metab., 30 (11). S. 1793 - 1804. NEW YORK: NATURE PUBLISHING GROUP. ISSN 0271-678X

Full text not available from this repository.

Abstract

The purpose of this study was to investigate hemoglobin and iron handling after subarachnoid hemorrhage (SAH), examine the relationship between iron and neuroglial cell changes, and determine whether deferoxamine (DFX) can reduce SAH-induced injury. The SAH was induced in Sprague-Dawley rats (n = 110) using an endovascular perforation technique. Animals were treated with DFX (100 mg/kg) or vehicle 2 and 6 hours after SAH induction followed by every 12 hours for 3 days. Rats were killed at 6 hours, Days 1 and 3 to determine nonheme iron and examine iron-handling proteins using Western blot and immunohistochemistry. 8-Hydroxyl-2'-deoxyguanosine and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining were performed to assess oxidative DNA damage and neuronal cell death. After SAH, marked heme-oxygenase-1 (HO-1) upregulation at Day 3 (P < 0.01) was accompanied by elevated nonheme iron (P < 0.01), transferrin (Tf) (P < 0.01), Tf receptor (P < 0.05), and ferritin levels (P < 0.01). Deferoxamine treatment reduced SAH-induced mortality (12% versus 29%, P < 0.05), brain nonheme iron concentration, iron-handling protein expression, oxidative stress, and neuronal cell death at Day 3 (P < 0.01) after SAH. These results suggest that iron overload in the acute phase of SAH causes oxidative injury leading to neuronal cell death. Deferoxamine effectively reduced oxidative stress and neuronal cell death, and may be a potential therapeutic agent for SAH. Journal of Cerebral Blood Flow & Metabolism (2010) 30, 1793-1803; doi:10.1038/jcbfm.2010.137; published online 25 August 2010

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lee, Jin-Yul UNSPECIFIED UNSPECIFIED UNSPECIFIED Keep, Richard F. UNSPECIFIED UNSPECIFIED UNSPECIFIED He, Yangdong UNSPECIFIED UNSPECIFIED UNSPECIFIED Sagher, Oren UNSPECIFIED UNSPECIFIED UNSPECIFIED Hua, Ya UNSPECIFIED UNSPECIFIED UNSPECIFIED Xi, Guohua UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-493515
DOI:	10.1038/jcbfm.2010.137
Journal or Publication Title:	J. Cereb. Blood Flow Metab.
Volume:	30
Number:	11
Page Range:	S. 1793 - 1804
Date:	2010
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	NEW YORK
ISSN:	0271-678X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPERIMENTAL INTRACEREBRAL HEMORRHAGE; CELL-DEATH; OXIDATIVE STRESS; HEME OXYGENASE-1; FERRITIN EXPRESSION; CEREBRAL-ISCHEMIA; RAT MODEL; AUTOPHAGY; NEURONS; EDEMA Multiple languages Endocrinology & Metabolism; Hematology; Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49351