Universität zu Köln

Shahriari, Mojgan, Keshavaiah, Channa, Scheuring, David ORCID: 0000-0001-9048-3330, Sabovljevic, Aneta, Pimpl, Peter ORCID: 0000-0001-5031-8672, Haeusler, Rainer E., Huelskamp, Martin and Schellmann, Swen (2010). The AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana. Plant J., 64 (1). S. 71 - 86. MALDEN: WILEY-BLACKWELL. ISSN 0960-7412

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Abstract

P>The vacuole is the most prominent organelle of plant cells. Despite its importance for many physiological and developmental aspects of plant life, little is known about its biogenesis and maintenance. Here we show that Arabidopsis plants expressing a dominant-negative version of the AAA (ATPase associated with various cellular activities) ATPase AtSKD1 (SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) under the control of the trichome-specific GLABRA2 (GL2) promoter exhibit normal vacuolar development in early stages of trichome development. Shortly after its formation, however, the large central vacuole is fragmented and finally disappears completely. Secretion assays with amylase fused to the vacuolar sorting signal of Sporamin show that dominant-negative AtSKD1 inhibits vacuolar trafficking of the reporter that is instead secreted. In addition, trichomes expressing dominant-negative AtSKD1 frequently contain multiple nuclei. Our results suggest that AtSKD1 contributes to vacuolar protein trafficking and thereby to the maintenance of the large central vacuole of plant cells, and might play a role in cell-cycle regulation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Shahriari, Mojgan UNSPECIFIED UNSPECIFIED UNSPECIFIED Keshavaiah, Channa UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheuring, David UNSPECIFIED orcid.org/0000-0001-9048-3330 UNSPECIFIED Sabovljevic, Aneta UNSPECIFIED UNSPECIFIED UNSPECIFIED Pimpl, Peter UNSPECIFIED orcid.org/0000-0001-5031-8672 UNSPECIFIED Haeusler, Rainer E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huelskamp, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schellmann, Swen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-496190
DOI:	10.1111/j.1365-313X.2010.04310.x
Journal or Publication Title:	Plant J.
Volume:	64
Number:	1
Page Range:	S. 71 - 86
Date:	2010
Publisher:	WILEY-BLACKWELL
Place of Publication:	MALDEN
ISSN:	0960-7412
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TISSUE-SPECIFIC EXPRESSION; MULTIVESICULAR BODY; SACCHAROMYCES-CEREVISIAE; PREVACUOLAR COMPARTMENT; MEMBRANE ASSOCIATION; PROTEIN INTERACTIONS; SECRETORY PATHWAY; ENDOSOME FUNCTION; MAMMALIAN-CELLS; ESCRT MACHINERY Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49619