Universität zu Köln

Pfannkuche, Kurt, Neuss, Sabine, Pillekamp, Frank, Frenzel, Lukas P., Attia, Wael ORCID: 0000-0001-5822-6481, Hannes, Tobias ORCID: 0000-0002-8550-2282, Salber, Jochen ORCID: 0000-0001-9738-2305, Hoss, Mareike, Zenke, Martin, Fleischmann, Bernd K., Hescheler, Juergen and Saric, Tomo ORCID: 0000-0001-8344-1095 (2010). Fibroblasts Facilitate the Engraftment of Embryonic Stem Cell-Derived Cardiomyocytes on Three-Dimensional Collagen Matrices and Aggregation in Hanging Drops. Stem Cells Dev., 19 (10). S. 1589 - 1600. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1557-8534

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Abstract

There is growing interest in the use of cardiomyocytes purified from embryonic stem (ES) cells for tissue engineering and cardiomyoplasty. However, most transplanted cells are lost shortly after transplantation due to the lack of integration into the host tissue and subsequent apoptosis. Here we examine whether murine embryonic fibroblasts (MEFs) can support the integration of purified murine ES cell-derived cardiomyocytes in a 3-dimensional tissue culture model based on a freezed-dryed collagen matrix with tubular structure. Collagen matrix was seeded either with cardiomyocytes alone or in combination with MEFs. The collagen sponges that were transplanted with cardiomyocytes alone showed neither morphological nor functional integration of viable cells. Cardiomyocytes also did not appear to be capable of attaching quantitatively to any of 16 different 2-dimensional biomaterials. However, cardiomyocytes co-cultured with MEFs formed fiber-like structures of rod-shaped cells with organized sarcomeric structure that contracted spontaneously. Electrical coupling between cardiomyocytes was suggested by strong expression of connexin 43. In addition, MEFs as well as cardiac fibroblasts supported re-aggregation of dissociated cardiomyocytes in hanging drops in the absence of collagen matrix. We conclude that fibroblasts promote cardiomyocyte engraftment and formation of functional 3-dimensional tissue in vitro. Elucidation of the mechanism of this phenomenon may help improve the integration of cardiomyocytes in vivo.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pfannkuche, Kurt UNSPECIFIED UNSPECIFIED UNSPECIFIED Neuss, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Pillekamp, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Frenzel, Lukas P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Attia, Wael UNSPECIFIED orcid.org/0000-0001-5822-6481 UNSPECIFIED Hannes, Tobias UNSPECIFIED orcid.org/0000-0002-8550-2282 UNSPECIFIED Salber, Jochen UNSPECIFIED orcid.org/0000-0001-9738-2305 UNSPECIFIED Hoss, Mareike UNSPECIFIED UNSPECIFIED UNSPECIFIED Zenke, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Fleischmann, Bernd K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED orcid.org/0000-0001-8344-1095 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-496269
DOI:	10.1089/scd.2009.0255
Journal or Publication Title:	Stem Cells Dev.
Volume:	19
Number:	10
Page Range:	S. 1589 - 1600
Date:	2010
Publisher:	MARY ANN LIEBERT, INC
Place of Publication:	NEW ROCHELLE
ISSN:	1557-8534
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IMPROVE CARDIAC-FUNCTION; ENGINEERED HEART-TISSUE; INFARCTED RAT HEARTS; MYOCARDIAL-INFARCTION; MUSCLE-CELLS; IN-VITRO; SURVIVAL; DIFFERENTIATION; MYOCYTES; GRAFTS Multiple languages Cell & Tissue Engineering; Hematology; Medicine, Research & Experimental; Transplantation Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49626