Universität zu Köln

Soehnchen, J., Grosheva, M., Kiryakova, S., Huebbers, C. U., Sinis, N., Skouras, E., Ankerne, J., Kaidoglou, K., Fries, J. W. U., Irintchev, A., Dunlop, S. A. and Angelov, D. N. (2010). RECOVERY OF WHISKING FUNCTION AFTER MANUAL STIMULATION OF DENERVATED VIBRISSAL MUSCLES REQUIRES BRAIN-DERIVED NEUROTROPHIC FACTOR AND ITS RECEPTOR TYROSINE KINASE B. Neuroscience, 170 (1). S. 372 - 381. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1873-7544

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Abstract

Functional recovery following facial nerve injury is poor. Neuromuscular junctions (NMJs) are bridged by terminal Schwann cells and numerous regenerating axonal sprouts. We have shown that this poly-innervation of NMJs can be reduced by manual stimulation (MS) with restoration of whisking function. In addition, we have recently reported that insulin-like growth factor-1 (IGF-1) is required to mediate the beneficial effects of MS. Here we extend our findings to brain derived neurotrophic factor (BDNF). We then examined the effect of MS after facial-facial anastomosis (FFA) in heterozygous mice deficient in BDNF (BDNF+/-) or in its receptor TrkB (TrkB(+/-)). We quantified vibrissal motor performance and the percentage of NMJ bridged by S100-positive terminal Schwann cells. In intact BONF+/- or TrkB(+/-) mice and their wild type (WT) littermates, there were no differences in vibrissal whisking nor in the percentage of bridged NMJ (0% in each genotype). After FFA and handling alone (i.e. no MS) in WT animals, vibrissal whisking amplitude was reduced (60% lower than intact) and the percentage of bridged NMJ increased (27% more than intact). MS improved both the amplitude of vibrissal whisking (not significantly different from intact) and the percentage of bridged NMJ (11% more than intact). After FFA and handling in BDNF+/- or TrkB(+/-) mice, whisking amplitude was again reduced (53% and 60% lower than intact) and proportion of bridged NMJ increased (24% and 29% more than intact). However, MS failed to improve outcome in both heterozygous strains (whisking amplitude 55% and 58% lower than intact; proportion of bridged NMJ 27% and 18% more than intact). We conclude that BDNF and TRkB are required to mediate the effects of MS on target muscle reinnervation and recovery of whisking function. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Soehnchen, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Grosheva, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kiryakova, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huebbers, C. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sinis, N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Skouras, E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ankerne, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaidoglou, K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, J. W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Irintchev, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dunlop, S. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Angelov, D. N. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-496322
DOI:	10.1016/j.neuroscience.2010.06.053
Journal or Publication Title:	Neuroscience
Volume:	170
Number:	1
Page Range:	S. 372 - 381
Date:	2010
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1873-7544
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FACIAL-NERVE INJURY; RAT SCIATIC-NERVE; NEURON REGENERATION ACCURACY; VENTRAL SPINAL-CORD; GROWTH-FACTOR; SCHWANN-CELLS; NEUROMUSCULAR-JUNCTION; PERIPHERAL-NERVE; SYNAPTIC PLASTICITY; ADULT-RAT Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49632