Universität zu Köln

Kalkhof, Stefan ORCID: 0000-0001-6121-7105, Witte, Konstanze, Ihling, Christian H., Mueller, Mathias Q., Keller, Manuel V., Haehn, Sebastian, Smyth, Neil ORCID: 0000-0002-3734-2149, Paulsson, Mats and Sinz, Andrea (2010). A Novel Disulfide Pattern in Laminin-Type Epidermal Growth Factor-like (LE) Modules of Laminin beta 1 and gamma 1 Chains. Biochemistry, 49 (38). S. 8359 - 8367. WASHINGTON: AMER CHEMICAL SOC. ISSN 0006-2960

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Abstract

In-depth mass spectrometric analysis of disulfide bond patterns in recombinant mouse laminin beta 1 and gamma 1 chain N-terminal fragments comprising the laminin N-terminal (LN) domain and the first four laminin epidermal growth factor-like (LE) domains revealed a novel disulfide pattern for LE domains. This showed a (2-3, 4-5, 6-7, 8-1) connectivity with the last cysteine of one LE domain being connected to the first cysteine of the following LE domain. The same pattern was also found in E4, the N-terminal beta 1 chain fragment derived by elastase digestion of mouse EHS tumor laminin-111, showing that this pattern occurs in native laminin. The strictly linear pattern with an interdomain disulfide has not been described previously for EGF domains. The N-terminal portions of laminin short arms, consisting of the LN domain and LE domains 1-4, are essential for laminin-laminin self-interactions, whereas the internal LE domains 7-9 in the laminin gamma 1 chain harbor the nidogen binding site and have a conventional disulfide pattern. This suggests that LE domains differing in function also differ in their disulfide patterns.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kalkhof, Stefan UNSPECIFIED orcid.org/0000-0001-6121-7105 UNSPECIFIED Witte, Konstanze UNSPECIFIED UNSPECIFIED UNSPECIFIED Ihling, Christian H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Mathias Q. UNSPECIFIED UNSPECIFIED UNSPECIFIED Keller, Manuel V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Haehn, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Smyth, Neil UNSPECIFIED orcid.org/0000-0002-3734-2149 UNSPECIFIED Paulsson, Mats UNSPECIFIED UNSPECIFIED UNSPECIFIED Sinz, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-496336
DOI:	10.1021/bi101187f
Journal or Publication Title:	Biochemistry
Volume:	49
Number:	38
Page Range:	S. 8359 - 8367
Date:	2010
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	0006-2960
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SITE-DIRECTED MUTAGENESIS; NIDOGEN BINDING-SITE; SOMATOMEDIN-B-DOMAIN; N-TERMINAL DOMAINS; MULTIDOMAIN PROTEIN; BASEMENT-MEMBRANES; CRYSTAL-STRUCTURE; BONDS; IDENTIFICATION; NOMENCLATURE Multiple languages Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49633