Universität zu Köln

Marten, Gernot U., Gelbrich, Thorsten and Schmidt, Annette M. (2010). Hybrid biofunctional nanostructures as stimuli-responsive catalytic systems. Beilstein J. Org. Chem., 6. S. 922 - 932. FRANKFURT AM MAIN: BEILSTEIN-INSTITUT. ISSN 1860-5397

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Abstract

A novel active biocatalytic reaction system is proposed by covalently immobilizing porcine pancreas trypsin within the thermoresponsive polymer shell of superparamagnetic Fe3O4 nanoparticles. Active ester-functional nanocarriers suitable for the immobilization of amino functional targets are obtained in a single polymerization step by grafting-from copolymerization of an active ester monomer from superparamagnetic cores. The comonomer, oligo(ethylene glycol) methyl ether methacrylate, has excellent water solubility at room temperature, biocompatibility, and a tunable lower critical solution temperature (LCST) in water. The phase separation can alternatively be initiated by magnetic heating caused by magnetic losses in ac magnetic fields. The immobilization of porcine pancreas trypsin to the core-shell nanoparticles results in highly active, nanoparticulate biocatalysts that can easily be separated magnetically. The enzymatic activity of the obtained biocatalyst system can be influenced by outer stimuli, such as temperature and external magnetic fields, by utilizing the LCST of the copolymer shell.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Marten, Gernot U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gelbrich, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Annette M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-496536
DOI:	10.3762/bjoc.6.98
Journal or Publication Title:	Beilstein J. Org. Chem.
Volume:	6
Page Range:	S. 922 - 932
Date:	2010
Publisher:	BEILSTEIN-INSTITUT
Place of Publication:	FRANKFURT AM MAIN
ISSN:	1860-5397
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IRON-OXIDE NANOPARTICLES; TRANSFER RADICAL POLYMERIZATION; MAGNETIC PARTICLE HYPERTHERMIA; GRAFTED SILICA PARTICLES; CORE-SHELL NANOPARTICLES; N-ISOPROPYLACRYLAMIDE; DRUG-DELIVERY; PARAMETERS; IMMOBILIZATION; PURIFICATION Multiple languages Chemistry, Organic Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49653