Universität zu Köln

Gupta, Manoj K., Illich, Damir J., Gaarz, Andrea, Matzkies, Matthias, Nguemo, Filomain, Pfannkuche, Kurt, Liang, Huamin, Classen, Sabine, Reppel, Michael, Schultze, Joachim L., Hescheler, Juergen and Saric, Tomo ORCID: 0000-0001-8344-1095 (2010). Global transcriptional profiles of beating clusters derived from human induced pluripotent stem cells and embryonic stem cells are highly similar. BMC Dev. Biol., 10. LONDON: BMC. ISSN 1471-213X

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Abstract

Background: Functional and molecular integrity of cardiomyocytes (CMs) derived from induced pluripotent stem (iPS) cells is essential for their use in tissue repair, disease modelling and drug screening. In this study we compared global transcriptomes of beating clusters (BCs) microdissected from differentiating human iPS cells and embryonic stem (ES) cells. Results: Hierarchical clustering and principal component analysis revealed that iPS-BCs and ES-BCs cluster together, are similarly enriched for cardiospecific genes and differ in expression of only 1.9% of present transcripts. Similarly, sarcomeric organization, electrophysiological properties and calcium handling of iPS-CMs were indistinguishable from those of ES-CMs. Gene ontology analysis revealed that among 204 genes that were upregulated in iPS-BCs vs ES-BCs the processes related to extracellular matrix, cell adhesion and tissue development were overrepresented. Interestingly, 47 of 106 genes that were upregulated in undifferentiated iPS vs ES cells remained enriched in iPS-BCs vs ES-BCs. Most of these genes were found to be highly expressed in fibroblasts used for reprogramming and 34% overlapped with the recently reported iPS cell-enriched genes. Conclusions: These data suggest that iPS-BCs are transcriptionally highly similar to ES-BCs. However, iPS-BCs appear to share some somatic cell signature with undifferentiated iPS cells. Thus, iPS-BCs may not be perfectly identical to ES-BCs. These minor differences in the expression profiles may occur due to differential cellular composition of iPS-BCs and ES-BCs, due to retention of some genetic profile of somatic cells in differentiated iPS cell-derivatives, or both.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gupta, Manoj K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Illich, Damir J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gaarz, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Matzkies, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Nguemo, Filomain UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfannkuche, Kurt UNSPECIFIED UNSPECIFIED UNSPECIFIED Liang, Huamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Classen, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Reppel, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Schultze, Joachim L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED orcid.org/0000-0001-8344-1095 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-496543
DOI:	10.1186/1471-213X-10-98
Journal or Publication Title:	BMC Dev. Biol.
Volume:	10
Date:	2010
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1471-213X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARDIAC MYOCYTES; HESC LINES; DIFFERENTIATION; GENERATION; EXPRESSION; CARDIOMYOCYTES; FIBROBLASTS; TISSUE; MICROARRAY; SIGNATURE Multiple languages Developmental Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49654