Universität zu Köln

Welker, Anton, Hennes, Marc, Bender, Niklas, Cronenberg, Tom, Schneider, Gabriele and Maier, Berenike ORCID: 0000-0001-6971-9927 (2021). Spatiotemporal dynamics of growth and death within spherical bacterial colonies. Biophys. J., 120 (16). S. 3418 - 3429. CAMBRIDGE: CELL PRESS. ISSN 1542-0086

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Abstract

Bacterial growth within colonies and biofilms is heterogeneous. Local reduction of growth rates has been associated with tolerance against various antibiotics. However, spatial gradients of growth rates are poorly characterized in three-dimensional bacterial colonies. Here, we report two spatially resolved methods for measuring growth rates in bacterial colonies. As bacteria grow and divide, they generate a velocity field that is directly related to the growth rates. We derive profiles of growth rates from the velocity field and show that they are consistent with the profiles obtained by single-cell-counting. Using these methods, we reveal that even small colonies initiated with a few thousand cells of the human pathogen Neisseria gonorrhoeae develop a steep gradient of growth rates within two generations. Furthermore, we show that stringent response decelerates growth inhibition at the colony center. Based on our results, we suggest that aggregation-related growth inhibition can protect gonococci from external stresses even at early biofilm stages.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Welker, Anton UNSPECIFIED UNSPECIFIED UNSPECIFIED Hennes, Marc UNSPECIFIED UNSPECIFIED UNSPECIFIED Bender, Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Cronenberg, Tom UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Maier, Berenike UNSPECIFIED orcid.org/0000-0001-6971-9927 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-562613
DOI:	10.1016/j.bpj.2021.06.022
Journal or Publication Title:	Biophys. J.
Volume:	120
Number:	16
Page Range:	S. 3418 - 3429
Date:	2021
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1542-0086
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ANTIBIOTIC TOLERANCE; BIOFILMS; ARCHITECTURE; (P)PPGPP; FLOW Multiple languages Biophysics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56261