Stephan, Lisa ORCID: 0000-0002-1296-7548, Jakoby, Marc, Das, Arijit
ORCID: 0000-0002-1108-6052, Koebke, Eva and Huelskamp, Martin
(2021).
Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.
PLoS One, 16 (5).
SAN FRANCISCO:
PUBLIC LIBRARY SCIENCE.
ISSN 1932-6203
Abstract
The directional movement and positioning of organelles and macromolecules is essential for regulating and maintaining cellular functions in eukaryotic cells. In plants, these processes are actin-based and driven by class XI myosins, which transport various cargos in a directed manner. As the analysis of myosin function is challenging due to high levels of redundancy, dominant negative acting truncated myosins have frequently been used to study intracellular transport processes. A comparison of the dominant negative effect of the coiled-coil domains and the GTD domains revealed a much stronger inhibition of P-body movement by the GTD domains. In addition, we show that the GTD domain does not inhibit P-body movement when driven by a hybrid myosin in which the GTD domain was replaced by DCP2. These data suggest that the dominant negative effect of myosin tails involves a competition of the GTD domains for cargo binding sites.
Item Type: | Journal Article | ||||||||||||||||||||||||
Creators: |
|
||||||||||||||||||||||||
URN: | urn:nbn:de:hbz:38-568273 | ||||||||||||||||||||||||
DOI: | 10.1371/journal.pone.0252327 | ||||||||||||||||||||||||
Journal or Publication Title: | PLoS One | ||||||||||||||||||||||||
Volume: | 16 | ||||||||||||||||||||||||
Number: | 5 | ||||||||||||||||||||||||
Date: | 2021 | ||||||||||||||||||||||||
Publisher: | PUBLIC LIBRARY SCIENCE | ||||||||||||||||||||||||
Place of Publication: | SAN FRANCISCO | ||||||||||||||||||||||||
ISSN: | 1932-6203 | ||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||
Uncontrolled Keywords: |
|
||||||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/56827 |
Downloads
Downloads per month over past year
Altmetric
Export
Actions (login required)
![]() |
View Item |