Universität zu Köln

Knoop, Andre ORCID: 0000-0003-0040-8754, Fusshoeller, Gregor, Haenelt, Nadine, Goergens, Christian, Guddat, Sven, Geyer, Hans and Thevis, Mario (2021). Mass spectrometric characterization of urinary hydrafinil metabolites for routine doping control purposes. Drug Test. Anal., 13 (11-12). S. 1915 - 1921. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

Little information on the human metabolism and urinary elimination of hydrafinil (9-fluorenol) exists. In order to support preventive anti-doping activities concerning compounds such as hydrafinil, a pilot elimination study was conducted with three healthy male volunteers receiving a single oral dose of 50 mg of hydrafinil. Urine samples were collected prior to and up to 72-h post-administration and were subjected to both gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, which allowed for the identification of the intact drug as well as Phase I and Phase II metabolites, primarily hydroxylated and/or glucuronidated or sulfo-conjugated hydrafinil. The identity of these metabolites was corroborated by high-resolution/high-accuracy tandem mass spectrometry, and the applicability of routine doping control workflows for the detection of hydrafinil and its main metabolites was assessed. Therefore, two findings of hydrafinil and its metabolites were recorded, which concerned out-of-competition doping control samples and, hence, were not pursued with confirmatory analyses. Yet, the initial testing procedure results indicate that hydrafinil might require consideration in sports drug testing programs to ensure its detection, if classified as prohibited by the World Anti-Doping Agency (WADA).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Knoop, Andre UNSPECIFIED orcid.org/0000-0003-0040-8754 UNSPECIFIED Fusshoeller, Gregor UNSPECIFIED UNSPECIFIED UNSPECIFIED Haenelt, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Goergens, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Guddat, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Geyer, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-571414
DOI:	10.1002/dta.3137
Journal or Publication Title:	Drug Test. Anal.
Volume:	13
Number:	11-12
Page Range:	S. 1915 - 1921
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MODAFINIL; EXPOSURE Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57141