Chen, Jing, Isachenko, Evgenia, Wang, Wanxue, Du, Xinxin, Wang, Mengying, Rahimi, Gohar, Mallmann, Peter and Isachenko, Vladimir ORCID: 0000-0002-3674-543X . Optimization of Follicle Isolation for Bioengineering of Human Artificial Ovary. Biopreserv. Biobank.. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1947-5543

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Abstract

Background: A functional artificial ovary is a promising strategy to recover fertility and restore endocrine function in cancer patients. The aim of this study is to optimize the follicle isolation protocol for cryopreserved human ovarian tissues.Methods: Each of the cryopreserved human ovarian cortex pieces (OCPs) from 10 patients was cut into two equal parts and randomly distributed into two treatment groups. Group 1: OCPs digested with Tumor Dissociation Enzyme (TDE); Group 2: OCPs digested with Liberase Dispase High (DH). The efficiency of both groups were evaluated in terms of yield, viability, morphology, and a short-term in vitro culture (IVC) in alginate scaffolds.Results: The TDE can isolate more primordial follicles and smaller diameter of follicles than Liberase DH. The TDE also enabled the isolation of more bright red follicles, higher percent of viable follicles, more morphologically normal follicles, and lower oxidative stress levels compared with Liberase DH. After eight days of IVC, follicles in the TDE group had a higher growth rate from Day 0 to Day 8, and higher viability on Day 8 than the Liberase DH Group.Conclusion: The TDE can be considered an alternative to Liberase DH, enables the isolation of a higher number of healthy follicles from human OCPs, and improves follicle survival after IVC in contrast to Liberase DH.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Chen, JingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, EvgeniaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, WanxueUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Du, XinxinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, MengyingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rahimi, GoharUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mallmann, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, VladimirUNSPECIFIEDorcid.org/0000-0002-3674-543XUNSPECIFIED
URN: urn:nbn:de:hbz:38-571933
DOI: 10.1089/bio.2021.0060
Journal or Publication Title: Biopreserv. Biobank.
Publisher: MARY ANN LIEBERT, INC
Place of Publication: NEW ROCHELLE
ISSN: 1947-5543
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PRE-ANTRAL FOLLICLES; LONG-TERM CULTURE; NITRIC-OXIDE; PRIMORDIAL FOLLICLES; PREANTRAL FOLLICLES; ENZYMATIC ISOLATION; CYTOKINE PRODUCTION; LUMINESCENCE ASSAY; IN-VITRO; GROWTHMultiple languages
Cell Biology; Chemistry, Applied; Medical Laboratory TechnologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/57193

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