Universität zu Köln

Murschall, Lena M., Peker, Esra, MacVicar, Thomas, Langer, Thomas and Riemer, Jan (2021). Protein Import Assay into Mitochondria Isolated from Human Cells. Bio-protocol, 11 (12). SUNNYVALE: BIO-PROTOCOL. ISSN 2331-8325

Full text not available from this repository.

Abstract

Mitochondria are essential organelles containing approximately 1,500 proteins. Only approximately 1% of these proteins are synthesized inside mitochondria, whereas the remaining 99% are synthesized as precursors on cytosolic ribosomes and imported into the organelle. Various tools and techniques to analyze the import process have been developed. Among them, in vitro reconstituted import systems are of importance to study these processes in detail. These experiments monitor the import reaction of mitochondria! precursors that were previously radiolabeled in a cell-free environment. However, the methods described have been mostly performed in mitochondria isolated from S. cerevisiae. Here, we describe the adaptation of this powerful assay to import proteins into crude mitochondria isolated from human tissue culture cells. [GRAPHICS] .

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Murschall, Lena M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Peker, Esra UNSPECIFIED UNSPECIFIED UNSPECIFIED MacVicar, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Langer, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Riemer, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-574125
DOI:	10.21769/BioProtoc.4057
Journal or Publication Title:	Bio-protocol
Volume:	11
Number:	12
Date:	2021
Publisher:	BIO-PROTOCOL
Place of Publication:	SUNNYVALE
ISSN:	2331-8325
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57412