Universität zu Köln

Seyerlein, Luisa, Gillard, Nathalie, Delahaut, Philippe ORCID: 0000-0003-2328-3042, Pierret, Gilles, Thomas, Andreas and Thevis, Mario (2021). Depletion of clomiphene residues in eggs and muscle after oral administration to laying hens. Food Addit. Contam. Part A-Chem., 38 (11). S. 1875 - 1883. ABINGDON: TAYLOR & FRANCIS LTD. ISSN 1944-0057

Full text not available from this repository.

Abstract

The selective oestrogen receptor modulator (SERM) clomiphene is therapeutically used to induce ovulation. While prohibited as a doping agent in sports, it is frequently detected in sports drug testing urine samples. Few reports exist on clomiphene's (illicit) use in the farming industry to increase the egg production rate of laying hens, which creates a risk that eggs as well as edible tissue of these hens contain residues of clomiphene. To investigate the potential transfer of clomiphene into eggs and muscle tissue, laying hens were orally administered with clomiphene citrate at 10 mg/day for 28 days. To determine clomiphene residues in eggs, chicken breast and chicken thigh, the target analyte was extracted from homogenised material with acetonitrile and subjected to ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. The test method reached a limit of quantification (LOQ) of 1 mu g/kg and was characterised concerning specificity, precision, trueness and linearity. Analyses were performed on whole egg, egg white and yolk separately, and chicken muscle from breast and thigh. Clomiphene was detectable in eggs two days after the beginning of the drug administration period. The drug concentrations increased to 10-20 mu g per egg within one week, and after withdrawal of clomiphene, residues decreased after 4 days, but traces of clomiphene were still detectable until the end of the study (14 days after the last administration). In the chicken's muscle tissue, clomiphene levels up to 150 mu g/kg (thigh) and 36 mu g/kg (breast) were found. Six days after the last dose, tissue clomiphene concentrations fell below the LOQ. Overall, these results underline the concerns that clomiphene may be transferred into animal-derived food and future research will therefore need to focus on assessing and minimising the risk of unintentional adverse analytical findings in doping controls.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Seyerlein, Luisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Gillard, Nathalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Delahaut, Philippe UNSPECIFIED orcid.org/0000-0003-2328-3042 UNSPECIFIED Pierret, Gilles UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-575379
DOI:	10.1080/19440049.2021.1949497
Journal or Publication Title:	Food Addit. Contam. Part A-Chem.
Volume:	38
Number:	11
Page Range:	S. 1875 - 1883
Date:	2021
Publisher:	TAYLOR & FRANCIS LTD
Place of Publication:	ABINGDON
ISSN:	1944-0057
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN CHORIONIC-GONADOTROPIN; FOOD CONTAMINATION; VETERINARY DRUGS; CITRATE; CLENBUTEROL; INDUCTION Multiple languages Chemistry, Applied; Food Science & Technology; Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57537