Krombholz, Sophia, Thomas, Andreas, Piper, Thomas, Lagojda, Andreas, Kuehne, Dirk and Thevis, Mario (2022). Urinary phenylethylamine metabolites as potential markers for sports drug testing purposes. Biomed. Chromatogr., 36 (2). HOBOKEN: WILEY. ISSN 1099-0801

Full text not available from this repository.

Abstract

The misuse of 2-phenylethylamine (PEA) in sporting competitions is prohibited by the World Anti-Doping Agency. As it is endogenously produced, a method is required to differentiate between naturally elevated levels of PEA and the illicit administration of the drug. In 2015, a sulfo-conjugated metabolite [2-(2-hydroxyphenyl)acetamide sulfate (M1)] was identified, and pilot study data suggested that the ratio M1/PEA could be used as a marker indicating the oral application of PEA. Within this project, the required reference material of M1 was synthesized, single and multiple dose elimination studies were conducted and 369 native urine samples of athletes were analyzed as a reference population. While the oral administration of only 100 mg PEA did not affect urinary PEA concentrations, an increase in urinary concentrations of M1 was observed for all volunteers. However, urinary concentrations of both PEA and M1 showed relatively large inter-individual differences and establishing a cut-off-level for M1/PEA proved difficult. Consequently, a second metabolite, phenylacetylglutamine, was considered. Binary logistic regression demonstrated a significant (P < 0.05) correlation of the urinary M1 and phenylacetylglutamine concentrations with an oral administration of PEA, suggesting that assessing both analytes can assist doping control laboratories in identifying PEA misuse.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Krombholz, SophiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thomas, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Piper, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lagojda, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuehne, DirkUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-575872
DOI: 10.1002/bmc.5274
Journal or Publication Title: Biomed. Chromatogr.
Volume: 36
Number: 2
Date: 2022
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1099-0801
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
DIETARY-SUPPLEMENTS; TRACE AMINES; ACID; PHENYLACETYLGLUTAMINE; CREATININE; MODULATOR; PLASMAMultiple languages
Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical; Pharmacology & PharmacyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/57587

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item