Universität zu Köln

Krombholz, Sophia, Thomas, Andreas, Piper, Thomas, Lagojda, Andreas, Kuehne, Dirk and Thevis, Mario (2022). Urinary phenylethylamine metabolites as potential markers for sports drug testing purposes. Biomed. Chromatogr., 36 (2). HOBOKEN: WILEY. ISSN 1099-0801

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Abstract

The misuse of 2-phenylethylamine (PEA) in sporting competitions is prohibited by the World Anti-Doping Agency. As it is endogenously produced, a method is required to differentiate between naturally elevated levels of PEA and the illicit administration of the drug. In 2015, a sulfo-conjugated metabolite [2-(2-hydroxyphenyl)acetamide sulfate (M1)] was identified, and pilot study data suggested that the ratio M1/PEA could be used as a marker indicating the oral application of PEA. Within this project, the required reference material of M1 was synthesized, single and multiple dose elimination studies were conducted and 369 native urine samples of athletes were analyzed as a reference population. While the oral administration of only 100 mg PEA did not affect urinary PEA concentrations, an increase in urinary concentrations of M1 was observed for all volunteers. However, urinary concentrations of both PEA and M1 showed relatively large inter-individual differences and establishing a cut-off-level for M1/PEA proved difficult. Consequently, a second metabolite, phenylacetylglutamine, was considered. Binary logistic regression demonstrated a significant (P < 0.05) correlation of the urinary M1 and phenylacetylglutamine concentrations with an oral administration of PEA, suggesting that assessing both analytes can assist doping control laboratories in identifying PEA misuse.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Krombholz, Sophia UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Piper, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Lagojda, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuehne, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-575872
DOI:	10.1002/bmc.5274
Journal or Publication Title:	Biomed. Chromatogr.
Volume:	36
Number:	2
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1099-0801
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DIETARY-SUPPLEMENTS; TRACE AMINES; ACID; PHENYLACETYLGLUTAMINE; CREATININE; MODULATOR; PLASMA Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57587